User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/10/15: Difference between revisions
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==SDS-PAGE: Running of the Gels== | ==SDS-PAGE: Running of the Gels== | ||
The four samples prepared yesterday were heated to 40C on a heating block. Afterwards, they were transferred into a premade gel along with a premade marker/ladder. The gel was then run under the same conditions as done earlier in the semester. | The four samples prepared yesterday were heated to 40C on a heating block. Afterwards, they were transferred into a premade gel along with a premade marker/ladder. The gel was then run under the same conditions as done earlier in the semester. (See notes from September 23, 2014) | ||
The following table depicts the placement of our samples in the 12-well gel: | The following table depicts the placement of our samples in the 12-well gel: |
Revision as of 05:07, 21 October 2014
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October 15, 2014Tornado WarningDisruptive... SDS-PAGE: Running of the GelsThe four samples prepared yesterday were heated to 40C on a heating block. Afterwards, they were transferred into a premade gel along with a premade marker/ladder. The gel was then run under the same conditions as done earlier in the semester. (See notes from September 23, 2014) The following table depicts the placement of our samples in the 12-well gel:
And the rest is history... Primary Experiment Dialysis #2 Work-up30:1 colloid solution vs CaCl2 using MWCO 3500
Necessary Analyses:
NOTE: There was a bit of a struggle getting a reliable spectrum for the blank. Was run 3 times in order to average/verify. We also made the executive decision to run samples of 50 μL from now on (diluted with 200 μL Bradford and 750 μL Tris/NaCl) in order to increase the precision/accuracy of the results.
Primary Experiment Dialysis #3 Set-Up0.6 g/L Lysozyme vs CaCl2 using MWCO 3500
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