User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/10/15: Difference between revisions

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(Autocreate 2014/10/15 Entry for User:Monika_Gasiorek/Notebook/CHEM-571_2014F)
 
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==Entry title==
==October 15, 2014==
* Insert content here...


=='''Tornado Warning'''==
Disruptive...


=='''Primary Experiment Dialysis #2 Work-up'''==
'''30:1 colloid solution vs CaCl2 using MWCO 3500'''
==<font color=#0f1028></font>==
{|style="width:700px"
|<u>'''Well 1'''</u>
|<u>'''Well 2'''</u>
|<u>'''Well 3'''</u>
|<u>'''Well 4'''</u>
|<u>'''Well 5'''</u>
|-
|30:1 colloid
|30:1 colloid
|30:1 colloid
|30:1 colloid
|30:1 colloid
|-
|50 mM CaCl<sub>2</sub>
|5 mM CaCl<sub>2</sub>
|0.5 mM CaCl<sub>2</sub>
|50 μM CaCl<sub>2</sub>
|5 μM CaCl<sub>2</sub>
|-
|}
'''Necessary Analyses:'''
*Bradford - completed, results need to be uploaded
**5 colloid samples, 30:1 colloid reference, and Bradford blank
**20 μL sample, 200 μL 1:4 Bradford, 780 μL 50 mM Tris/50 mM NaCl
'''NOTE''': There was a bit of a struggle getting a reliable spectrum for the blank. Was run 3 times in order to average/verify. We also made the executive decision to run samples of 50 μL from now on (diluted with 200 μL Bradford and 750 μL Tris/NaCl) in order to increase the precision/accuracy of the results.
*Fluorescence - not completed
**5 colloid samples, 30:1 colloid reference
**10 μL of sample in 990 μL of DI water
*UV-Vis - not completed
**5 colloid samples, 30:1 colloid reference
**10 μL of sample in 990 μL of DI water
*CaCl<sub>2</sub> ISE - not completed
**All 10 samples
=='''Primary Experiment Dialysis #3 Set-Up'''==
'''0.6 g/L Lysozyme vs CaCl2 using MWCO 3500'''
==<font color=#0f1028></font>==
{|style="width:700px"
|<u>'''Well 1'''</u>
|<u>'''Well 2'''</u>
|<u>'''Well 3'''</u>
|<u>'''Well 4'''</u>
|<u>'''Well 5'''</u>
|-
|0.6 g/L lysozyme
|0.6 g/L lysozyme
|0.6 g/L lysozyme
|0.6 g/L lysozyme
|0.6 g/L lysozome
|-
|50 mM CaCl<sub>2</sub>
|5 mM CaCl<sub>2</sub>
|0.5 mM CaCl<sub>2</sub>
|50 μM CaCl<sub>2</sub>
|5 μM CaCl<sub>2</sub>
|-
|}
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Revision as of 20:16, 15 October 2014

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October 15, 2014

Tornado Warning

Disruptive...

Primary Experiment Dialysis #2 Work-up

30:1 colloid solution vs CaCl2 using MWCO 3500

Well 1 Well 2 Well 3 Well 4 Well 5
30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid
50 mM CaCl2 5 mM CaCl2 0.5 mM CaCl2 50 μM CaCl2 5 μM CaCl2

Necessary Analyses:

  • Bradford - completed, results need to be uploaded
    • 5 colloid samples, 30:1 colloid reference, and Bradford blank
    • 20 μL sample, 200 μL 1:4 Bradford, 780 μL 50 mM Tris/50 mM NaCl

NOTE: There was a bit of a struggle getting a reliable spectrum for the blank. Was run 3 times in order to average/verify. We also made the executive decision to run samples of 50 μL from now on (diluted with 200 μL Bradford and 750 μL Tris/NaCl) in order to increase the precision/accuracy of the results.

  • Fluorescence - not completed
    • 5 colloid samples, 30:1 colloid reference
    • 10 μL of sample in 990 μL of DI water
  • UV-Vis - not completed
    • 5 colloid samples, 30:1 colloid reference
    • 10 μL of sample in 990 μL of DI water
  • CaCl2 ISE - not completed
    • All 10 samples

Primary Experiment Dialysis #3 Set-Up

0.6 g/L Lysozyme vs CaCl2 using MWCO 3500

Well 1 Well 2 Well 3 Well 4 Well 5
0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozome
50 mM CaCl2 5 mM CaCl2 0.5 mM CaCl2 50 μM CaCl2 5 μM CaCl2


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