User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/10/14: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* The four samples were placed in a heating block (set at 90 °C) for 5 minutes and stored in the fridge overnight | * The four samples were placed in a heating block (set at 90 °C) for 5 minutes and stored in the fridge overnight | ||
* | |||
*50 mL of a 66 mM Potassium Phosphate buffer with pH 6.24 was prepared using HPLC water, monobasic and dibasic potassium phosphate solids, KHPO<sub>4</sub> and K<sub>2</sub>HPO<sub>4</sub> | |||
=='''Primary Experiment Dialysis #1 Work-up'''== | =='''Primary Experiment Dialysis #1 Work-up'''== | ||
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'''Necessary Analyses:''' | '''Necessary Analyses:''' | ||
*Bradford - completed | *Bradford - completed | ||
**5 lysozyme samples, 0.6 g/L lysozyme reference, and Bradford blank | **5 lysozyme samples, 0.6 g/L lysozyme reference, and Bradford blank | ||
**20 μL sample, 200 μL 1:4 Bradford, 780 μL 50 mM Tris/50 mM NaCl | **20 μL sample, 200 μL 1:4 Bradford, 780 μL 50 mM Tris/50 mM NaCl | ||
*Fluorescence - completed | *Fluorescence - completed | ||
**5 lysozyme samples, 0.6 g/L lysozyme reference | **5 lysozyme samples, 0.6 g/L lysozyme reference | ||
**10 μL of sample in 990 μL of DI water | **10 μL of sample in 990 μL of DI water | ||
*UV-Vis - completed | *UV-Vis - completed | ||
**5 lysozyme samples, 0.6 g/L lysozyme reference | **5 lysozyme samples, 0.6 g/L lysozyme reference | ||
**10 μL of sample in 990 μL of DI water | **10 μL of sample in 990 μL of DI water | ||
*KI - | *KI - completed; see 10/21 for results | ||
**10 samples for titration with NH<sub>4</sub>SCN | **10 samples for titration with NH<sub>4</sub>SCN | ||
'''Bradford Results''' | |||
[[Image:Bradford_Lysozyme_KI_10_14_MGPR.png|500px]] | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Wavelength''' | |||
| align="center" style="background:#f0f0f0;"|''' Abs at 577 nm''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration (ug/mL)''' | |||
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| Stock||0.121||1.169902913 | |||
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| 2 mM||0.115||0.878640777 | |||
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| 5 mK||0.124||1.315533981 | |||
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| 10 mM||0.114||0.830097087 | |||
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| 25 mM||0.111||0.684466019 | |||
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| 50 mM||0.115||0.878640777 | |||
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'''Fluorescence Results''' | |||
[[Image:Lysozyme_KI_3500_Fluorescence_Emission_Chart.png|500px]] | |||
'''UV-VIS Results''' | |||
[[Image:Lysozyme_KI_3500MW_UV_VIS_1in100_Chart.png|500px]] | |||
=='''Primary Experiment Dialysis #2 Set-up'''== | =='''Primary Experiment Dialysis #2 Set-up'''== |
Latest revision as of 00:26, 27 September 2017
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October 14, 2014Preparation for SDS-PAGE - Take 2
Primary Experiment Dialysis #1 Work-up0.6 g/L Lysozyme vs KI using MWCO 3500
Necessary Analyses:
Bradford Results
Fluorescence Results UV-VIS Results Primary Experiment Dialysis #2 Set-up30:1 colloid solution vs CaCl2 using MWCO 3500
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