User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/08/27: Difference between revisions

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We then made 50 mL of a 250 μM solution of lysozyme.
We then made 50 mL of a 250 μM solution of lysozyme.
*0.179 g of solid lysozyme in 50 mL volumetric flask
*0.179 g of solid lysozyme in 50 mL volumetric flask
Finally, we made 50 mL of a 2.512 x 10<sup>-4</sup> M solution of HCl.
*25 μL of 0.5 M HCl was added to 49.975 mL of H<sub>2</sub>O
'''NOTE''': this needs to be re-done to match the concentration of the HAuCl<sub>4</sub> stock, or 2.512 mM.


6 culture tubes were prepared using the stock solutions to observe colloid versus fiber formation given different ratios of [Au]:[protein].
6 culture tubes were prepared using the stock solutions to observe colloid versus fiber formation given different ratios of [Au]:[protein].
The table below demonstrates the amounts of each stock solution and water added to 6 test tubes, each containing 5 mL of solution total.
The table below demonstrates the amounts of each stock solution and water added to 6 test tubes, each containing 5 mL of solution total.
==Kinetics of AuNP Formation==
*A 150 mL beaker containing approximately 100 mL of water was heated to 80°C.
*3 culture tubes were prepared each containing 0.5 mL of 2.512 mM HAuCl<sub>4</sub>, 0.167 mL of 2.512 x 10<sup>-4</sup> M lysozyme, and 4.333 mL of H<sub>2</sub>O, giving a [Au]:[protein] of 30:1. The test tubes were covered with foil and placed in the beaker of 80°C water.
*2.5 mL of one sample was placed in a plastic cuvette at time point 0 hr, pre-heating, to set a baseline UV-Vis spectrum from 800 nm to 360 nm.
*Another 2.5 mL aliquot of the samples was taken every 0.5 hrs and a respective UV-Vis was recorded from 800 nm to 360 nm until no more sample solution remained.
*Spectra were ultimately obtained at time-points 0, 0.5, 1, 1.5, 2, 2.5 hrs.


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Revision as of 18:45, 27 August 2014

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Preparation of Stock Solutions & Culture Tubes

We made 50 mL of a 2.512 mM solution of HAuCl4.

  • 0.043 g of solid HAuCl4 in 50 mL volumetric flask

We then made 50 mL of a 250 μM solution of lysozyme.

  • 0.179 g of solid lysozyme in 50 mL volumetric flask

Finally, we made 50 mL of a 2.512 x 10-4 M solution of HCl.

  • 25 μL of 0.5 M HCl was added to 49.975 mL of H2O

NOTE: this needs to be re-done to match the concentration of the HAuCl4 stock, or 2.512 mM.

6 culture tubes were prepared using the stock solutions to observe colloid versus fiber formation given different ratios of [Au]:[protein]. The table below demonstrates the amounts of each stock solution and water added to 6 test tubes, each containing 5 mL of solution total.

Kinetics of AuNP Formation

  • A 150 mL beaker containing approximately 100 mL of water was heated to 80°C.
  • 3 culture tubes were prepared each containing 0.5 mL of 2.512 mM HAuCl4, 0.167 mL of 2.512 x 10-4 M lysozyme, and 4.333 mL of H2O, giving a [Au]:[protein] of 30:1. The test tubes were covered with foil and placed in the beaker of 80°C water.
  • 2.5 mL of one sample was placed in a plastic cuvette at time point 0 hr, pre-heating, to set a baseline UV-Vis spectrum from 800 nm to 360 nm.
  • Another 2.5 mL aliquot of the samples was taken every 0.5 hrs and a respective UV-Vis was recorded from 800 nm to 360 nm until no more sample solution remained.
  • Spectra were ultimately obtained at time-points 0, 0.5, 1, 1.5, 2, 2.5 hrs.


NOTE: The amount of Au in each tube was intended to be 0.5 mL, or 1/10 the volume of the final solution. Using a stock solution with a [H+] of 2.512 mM, the concentration of [H+] in the final 5 mL solution would be 2.512 x 10-4. This would give a solution of 3.6. Because we used 2.5 mL of Au in each tube, or 1/2 the volume of the final solution, our culture tubes contained a higher [H+] and therefore had a lower pH of 2.9.

Each test tube was covered in foil and placed in an oven at 80°C for 3 hours.
Au Lysozyme H2O [Au]:[lysozyme]
2.5 mL 2.5 mL 0 mL 10:1
2.5 mL 1.25 mL 1.25 mL 20:1
2.5 mL 0.835 mL 1.665 mL 30:1
2.5 mL 0.625 mL 1.875 mL 40:1
2.5 mL 0.5 mL 2 mL 50:1
2.5 mL 0.415 mL 2.08 mL 60:1


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