User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/08/27: Difference between revisions
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==August 27, 2014== | |||
==Preparation of Stock Solutions & Culture Tubes== | ==Preparation of Stock Solutions & Culture Tubes== | ||
We made 50 mL of a 2.512 mM solution of HAuCl<sub>4</sub>. | We made 50 mL of a 2.512 mM solution of HAuCl<sub>4</sub>. | ||
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6 culture tubes were prepared using the stock solutions to observe colloid versus fiber formation given different ratios of [Au]:[protein]. | 6 culture tubes were prepared using the stock solutions to observe colloid versus fiber formation given different ratios of [Au]:[protein]. | ||
The table below demonstrates the amounts of each stock solution and water added to 6 test tubes, each containing 5 mL of solution total. | The table below demonstrates the amounts of each stock solution and water added to 6 test tubes, each containing 5 mL of solution total. | ||
==<font color=#0f1028></font>== | ==<font color=#0f1028></font>== | ||
{|style="width:700px" | {|style="width:700px" | ||
|<u>Au</u> | |<u>'''Au (mL)'''</u> | ||
|<u>Lysozyme</u> | |<u>'''Lysozyme (mL)'''</u> | ||
|<u>H<sub>2</sub>O</u> | |<u>'''H<sub>2</sub>O (mL)'''</u> | ||
|<u>[Au]:[lysozyme]</u> | |<u>'''[Au]:[lysozyme]'''</u> | ||
|- | |- | ||
|2.5 | |2.5 | ||
|2.5 | |2.5 | ||
|0 | |0 | ||
|10:1 | |10:1 | ||
|- | |- | ||
|2.5 | |2.5 | ||
|1.25 | |1.25 | ||
|1.25 | |1.25 | ||
|20:1 | |20:1 | ||
|- | |- | ||
|2.5 | |2.5 | ||
|0.835 | |0.835 | ||
|1.665 | |1.665 | ||
|30:1 | |30:1 | ||
|- | |- | ||
|2.5 | |2.5 | ||
|0.625 | |0.625 | ||
|1.875 | |1.875 | ||
|40:1 | |40:1 | ||
|- | |- | ||
|2.5 | |2.5 | ||
|0.5 | |0.5 | ||
|2 | |2 | ||
|50:1 | |50:1 | ||
|- | |- | ||
|2.5 | |2.5 | ||
|0.415 | |0.415 | ||
|2.08 | |2.08 | ||
|60:1 | |60:1 | ||
|- | |} | ||
'''NOTE''': The amount of Au in each tube was intended to be 0.5 mL, or 1/10 the volume of the final solution. Using a stock solution with a [H<sup>+</sup>] of 2.512 mM, the concentration of [H<sup>+</sup>] in the final 5 mL solution would be 2.512 x 10<sup>-4</sup>. This would give a solution of 3.6. Because we used 2.5 mL of Au in each tube, or 1/2 the volume of the final solution, our culture tubes contained a higher [H<sup>+</sup>] and therefore had a lower pH of 2.9. | |||
Each test tube was covered in foil and placed in an oven at 80°C for 3 hours. | |||
==Kinetics of AuNP Formation== | |||
*A 150 mL beaker containing approximately 100 mL of water was heated to 80°C. | |||
*3 culture tubes were prepared each containing 0.5 mL of 2.512 mM HAuCl<sub>4</sub>, 0.167 mL of 2.512 x 10<sup>-4</sup> M lysozyme, and 4.333 mL of H<sub>2</sub>O, giving a [Au]:[protein] of 30:1. The test tubes were covered with foil and placed in the beaker of 80°C water. | |||
*2.5 mL of one sample was placed in a plastic cuvette at time point 0 hr, pre-heating, to set a baseline UV-Vis spectrum from 800 nm to 360 nm. | |||
*Another 2.5 mL aliquot of the samples was taken every 0.5 hrs and a respective UV-Vis was recorded from 800 nm to 360 nm until no more sample solution remained. | |||
*Spectra were ultimately obtained at time-points 0, 0.5, 1, 1.5, 2, 2.5 hrs. | |||
[[Image:Kinetics_80.png]] | |||
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Latest revision as of 00:12, 27 September 2017
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August 27, 2014Preparation of Stock Solutions & Culture TubesWe made 50 mL of a 2.512 mM solution of HAuCl4.
We then made 50 mL of a 250 μM solution of lysozyme.
Finally, we made 50 mL of a 2.512 x 10-4 M solution of HCl.
NOTE: this needs to be re-done to match the concentration of the HAuCl4 stock, or 2.512 mM. 6 culture tubes were prepared using the stock solutions to observe colloid versus fiber formation given different ratios of [Au]:[protein]. The table below demonstrates the amounts of each stock solution and water added to 6 test tubes, each containing 5 mL of solution total.
NOTE: The amount of Au in each tube was intended to be 0.5 mL, or 1/10 the volume of the final solution. Using a stock solution with a [H+] of 2.512 mM, the concentration of [H+] in the final 5 mL solution would be 2.512 x 10-4. This would give a solution of 3.6. Because we used 2.5 mL of Au in each tube, or 1/2 the volume of the final solution, our culture tubes contained a higher [H+] and therefore had a lower pH of 2.9. Each test tube was covered in foil and placed in an oven at 80°C for 3 hours. Kinetics of AuNP Formation
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