User:Moira M. Esson/Notebook/CHEM-581/2013/04/12: Difference between revisions
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==X-Ray Analysis== | ==X-Ray Analysis== | ||
General Protocol: | |||
# Use a Kimwipe and acetone to thoroughly clean the low background sample holder | |||
# Insert Sample into the low background holder. Note: Must make sure that the sample is completely flat and that the particle sample size is very small. If the sample is too large, use a mortar and pestle to completely grind the sample | |||
# Turn on the chiller | |||
# Turn on Miniflex II. This is the white button on the machine | |||
# Turn on X-ray. The machine will be on when the red lights on the button labeled x-ray light up | |||
# Using a Geiger counter, test the radiation from the machine | |||
# Set sample in sample holder and record sample holder number | |||
# Close the door to the x-ray | |||
# Open software on computer | |||
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*50:50 PVA 130K:110%LP was run on the x-ray. The data will be collected during the next session. | |||
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==Hydrogel DSC preparation== | |||
*3mL of deionized H<sub>2</sub>O was added to each of the hydrogel samples. H<sub>2</sub>O was added in order to soften the hydrogels to allow DSC sample preparation. H<sub>2</sub>O was also added in order to remove any excess Rhodamine 6G still present. | |||
*The hydrogels will soak in deionized H<sub>2</sub>O until the next session. The water will be changed each day in order to remove the most Rhodamine 6G. | |||
==Microscope== | |||
* | |||
==Notes== | |||
*A plain oil sample will need to be run on the x-ray as a reference. Because of the presence of safflower oil in the prepared microsphere samples, the oil peak will be determined using the control oil spectrum. | |||
Revision as of 10:19, 15 April 2013
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Objectives
DSC
# Equilibrate sample at ~ -40°C # Ramp sample temperature up from 20-240°C # Ramp sample temperature down from 20-(-40°C) # Repeat segment #2 again
Film Preparation
X-Ray AnalysisGeneral Protocol:
Hydrogel DSC preparation
MicroscopeNotes
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