User:Moira M. Esson/Notebook/CHEM-581/2013/04/12: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio''' | | align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio''' | ||
| align="center" style="background:#f0f0f0;"|'''Clay Selection''' | | align="center" style="background:#f0f0f0;"|'''Clay Selection''' | ||
| align="center" style="background:#f0f0f0;"|'''PVOH | | align="center" style="background:#f0f0f0;"|'''PVOH 130K Mass (g)''' | ||
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)''' | | align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)''' | ||
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)''' | | align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)''' | ||
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|} | |} | ||
<br> | <br> | ||
==X-Ray Analysis== | ==X-Ray Analysis== | ||
General Protocol: | General Protocol: | ||
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# Close the door to the x-ray | # Close the door to the x-ray | ||
# Open software on computer | # Open software on computer | ||
*50:50 PVA 130K:110%LP was run on the x-ray. The data will be collected during the next session. | *50:50 PVA 130K:110%LP was run on the x-ray. The data will be collected during the next session. | ||
==Hydrogel DSC preparation== | ==Hydrogel DSC preparation== | ||
*3mL of deionized H<sub>2</sub>O was added to each of the hydrogel samples. H<sub>2</sub>O was added in order to soften the hydrogels to allow DSC sample preparation. H<sub>2</sub>O was also added in order to remove any excess Rhodamine 6G still present. | *3mL of deionized H<sub>2</sub>O was added to each of the hydrogel samples. H<sub>2</sub>O was added in order to soften the hydrogels to allow DSC sample preparation. H<sub>2</sub>O was also added in order to remove any excess Rhodamine 6G still present. | ||
*The hydrogels will soak in deionized H<sub>2</sub>O until the next session. The water will be changed each day in order to remove the most Rhodamine 6G. | *The hydrogels will soak in deionized H<sub>2</sub>O until the next session. The water will be changed each day in order to remove the most Rhodamine 6G. | ||
==Microscope== | ==Microscope== | ||
* | * In order to determine if true microspheres had been prepared, it was necessary to view the prepared microspheres under a microscope. | ||
* To obtain true microspheres, the prepared samples were sonicated with a high power-probe sonicator. | |||
<br> | |||
General Protocol: | |||
# Remove microsphere samples from vial. Place microsphere samples in a clean, 50mL falcon tube. | |||
# Add 10mL deionized H<sub>2</sub>O to the tube. | |||
# Sonicate the microspheres using the high power probe sonicator for 3 minutes. | |||
'''Note''': Use protective ear equiptment whenever opearting the high power sonicator | |||
# Once the microspheres are completely mixed(the solution should be a milk white color), add a small amount of sample onto a glass slide using a Pasteur pipette. | |||
# View microspheres starting on the lowest magnification setting. | |||
<br> | |||
* Two microsphere samples were viewed under the microscope: PVA MW130,000 and 50:50 PVA 130,000:110% LP | |||
* When viewed, both samples contained microspheres. | |||
==Notes== | ==Notes== | ||
*A plain oil sample will need to be run on the x-ray as a reference. Because of the presence of safflower oil in the prepared microsphere samples, the oil peak will be determined using the control oil spectrum. | *A plain oil sample will need to be run on the x-ray as a reference. Because of the presence of safflower oil in the prepared microsphere samples, the oil peak will be determined using the control oil spectrum. |
Revision as of 07:13, 19 April 2013
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Objectives
DSC
# Equilibrate sample at ~ -40°C # Ramp sample temperature up from 20-240°C # Ramp sample temperature down from 20-(-40°C) # Repeat segment #2 again
Film Preparation
X-Ray AnalysisGeneral Protocol:
Hydrogel DSC preparation
Microscope
Note: Use protective ear equiptment whenever opearting the high power sonicator
Notes
|