User:Moira M. Esson/Notebook/CHEM-581/2013/03/01: Difference between revisions

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   '''Figure 1. Fluorescence of hydrogel samples modified with Lamponite clay using an unmodified pipette for pressure testing'''
   '''Figure 1. Fluorescence of hydrogel samples modified with Lamponite clay using an unmodified pipette for pressure testing'''
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[[Image:Fluorescence of Rhodamine 6G collected from pressure test of hydrogels with Lamponite clay and using an unmodified pipette.png]]
   '''Figure 2. Fluorescence of hydrogel samples modified with NaMT clay using an unmodified pipette for pressure testing'''
   '''Figure 2. Fluorescence of hydrogel samples modified with NaMT clay using an unmodified pipette for pressure testing'''
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Notes:
Notes:
*The amount of dye, or concentration of dye, that was diffused from the hydrogels using an unmodified pipette was significantly lower than that of a bent pipette.
*The amount of dye, or concentration of dye, that was diffused from the hydrogels using an unmodified pipette was significantly lower than that of a bent pipette.
*The general trends observed using a bent pipette were also observed with an unmodified pipette.
*The general trends observed using a bent pipette were also observed with an unmodified pipette.(Unmodified clay had the highest absorbance values. On average, hydrogels with NaMT clays had a higher absorbance than the hydrogels prepared with lamponite clays. Similarly, the higher molecular weight PVA hydrogels exhibited higher absorbance values, indicating these hydrogels are better at exerting/responding to sheer pressure).
*Similar to the bent pipette, the concentration of Rhodamine 6G in the samples is extremely low in comparison to the 0.25μM Rhodamine 6G control, with the highest absorbance value of 9.204 observed in the 50:50 ratio of PVA MW 130,000:NaMT in comparison to the highest absorbance value of 606.17999 in the Rhodamine 6G 0.25μM. Another procedure that will exert more sheer pressure will be determined. A new method for the preparation of hydrogels will be considered where a single layer of clay will be incorporated into the hydrogel rather than having clay distributed throughout the hydrogel. 
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Revision as of 21:59, 7 March 2013

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Objectives

  1. Conduct pressure tests on all hydrogels using an unmodified pipette.
  2. Run fluorescence on all samples collected from the pressure tests.
  3. Decant the safflower oil from the microsphere samples prepared on 2013/02/22.


Pressure tests using an unmodified pipette

  • All pressure tests were conducted following the general protocol described on 2013/02/20.
  • Pressure tests were conducted using an unmodified pipette in order to determine if the very slight amount of sheer pressure caused by an unmodified pipette would cause a detectable amount of dye diffusion.


Table 1. Information concerning the pressure testing involving hydrogels with an unmodified pipette.

Sample Order PVOH vs. Clay Ratio PVOH Type Clay Selection Amount of Hydrogel Used (g)
1 50:50 130K 110% CEC NaMT w/ DMHXLBR 0.1006
2 50:50 130K 110% CEC NaMT w/ DMHXLBR 0.1022
3 90:10 130K Laponite 0.1031
4 50:50 146K 110% CEC Laponite w/ DMHXLBR 0.1066
5 90:10 130K 110% CEC Laponite w/ DMHXLBR 0.1083
6 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1003
7 50:50 130K 110% CEC Laponite w/ DMHXLBR 0.1036
8 50:50 146K Laponite 0.1016
9 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1011
10 90:10 146K 110% CEC NaMT w/ DMHXLBR 0.1020
11 50:50 130K NaMT 0.1024
12 50:50 130K 50% CEC NaMT w/ Bu3HdP+ 0.1015
13 50:50 146K 110% CEC NaMT w/ DMHXLBR 0.0998
14 90:10 130K 50% CEC NaMT w/ Bu3HdP+ 0.1026 


 Figure 1. Fluorescence of hydrogel samples modified with Lamponite clay using an unmodified pipette for pressure testing



 Figure 2. Fluorescence of hydrogel samples modified with NaMT clay using an unmodified pipette for pressure testing




 Figure 3. Fluorescence of all hydrogel samples using and unmodified pipette for pressure testing


Notes:

  • The amount of dye, or concentration of dye, that was diffused from the hydrogels using an unmodified pipette was significantly lower than that of a bent pipette.
  • The general trends observed using a bent pipette were also observed with an unmodified pipette.(Unmodified clay had the highest absorbance values. On average, hydrogels with NaMT clays had a higher absorbance than the hydrogels prepared with lamponite clays. Similarly, the higher molecular weight PVA hydrogels exhibited higher absorbance values, indicating these hydrogels are better at exerting/responding to sheer pressure).
  • Similar to the bent pipette, the concentration of Rhodamine 6G in the samples is extremely low in comparison to the 0.25μM Rhodamine 6G control, with the highest absorbance value of 9.204 observed in the 50:50 ratio of PVA MW 130,000:NaMT in comparison to the highest absorbance value of 606.17999 in the Rhodamine 6G 0.25μM. Another procedure that will exert more sheer pressure will be determined. A new method for the preparation of hydrogels will be considered where a single layer of clay will be incorporated into the hydrogel rather than having clay distributed throughout the hydrogel.


Microspheres

  • After the freeze



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