User:Moira M. Esson/Notebook/CHEM-581/2013/02/15: Difference between revisions
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Spectra: | Spectra: | ||
Figure 1. Diffusion test for 50:50 PVA MW 146,000-186,00: 50% NaMT hydrogel | Figure 1. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,00: 50% NaMT hydrogel | ||
[[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]] | [[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]] | ||
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Figure 2. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,000: NaMT hydrogel | |||
[[Image:2 hr diffusion test 50 MW 146 NaMT.png]] | |||
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Figure 3. Diffusion test fluorescence spectra for | |||
Observations: | Observations: | ||
*Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method. | *Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method. |
Revision as of 19:43, 6 March 2013
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Objectives
Microsphere Preparation
General Protocol:
For a 90:10 ratio: (25mL)(1μM)/(92μM)=0.272mL For a 50:50 ratio: (25mL)(1μM)/(165μM)=0.152mL
Fluorescence
General Protocol:
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