User:Moira M. Esson/Notebook/CHEM-581/2013/02/15: Difference between revisions
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General Protocol: | General Protocol: | ||
# The desired ratio of PVA and clay was measured out. The total mass was ~1g. | # The desired ratio of PVA and clay was measured out. The total mass was ~1g. | ||
# The PVA/clay was placed in a 50mL beaker with a magnetic stir bar. 25mL distilled H<sub>2</sub>O were added to the beaker and the solution was heated to ~100°C and allowed to stir until complete dissolution of PVA/clay. | |||
# The magnetic stir bar was removed and 25mL of mineral oil was added to the beaker. | |||
# The contents of the beaker were poured into a blender to homogenize the solution and create an emulsion of the aqueous and organic layer in the attempt to create a suspension of microspheres. | |||
# The blender was turned on a low setting for 5 minutes. | |||
# The contents of the blender were poured into a beaker and the appropriate amount of DMSO/Rhodamine 6G solution was added. | |||
# The microsphere solution was placed in a freezer at -20°C for 24 hours and then removed and allowed to thaw for 24 hours. | |||
# Repeat this freeze-thaw cycle three times. | |||
Preparation of Microspheres: | |||
*New calculations for the amount of DMSO/Rhodamine to be added: | |||
For a 90:10 ratio: | |||
(25mL)(1μM)/(92μM)=0.272mL | |||
For a 50:50 ratio: | |||
(25mL)(1μM)/(165μM)=0.152mL | |||
*Information about prepared microspheres: | |||
Revision as of 17:18, 17 February 2013
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Objectives
Microsphere Preparation
General Protocol:
Preparation of Microspheres:
For a 90:10 ratio: (25mL)(1μM)/(92μM)=0.272mL For a 50:50 ratio: (25mL)(1μM)/(165μM)=0.152mL
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