User:Moira M. Esson/Notebook/CHEM-581/2013/02/13: Difference between revisions
From OpenWetWare
Line 43: | Line 43: | ||
Spectra Obtained: | Spectra Obtained: | ||
<br> | <br> | ||
Figure 1. Diffusion test for hydrogel prepared as a 90:10 ratio of PVA MW 146,000:110%LP | |||
<br> | |||
[[Image:Diffusion test 90 MW 146 110%LP.png]] | |||
<br> | |||
Figure 2. Diffusion test for hydrogel prepared as | |||
==General Observations== | ==General Observations== | ||
*Further tests will need to be done concerning 110% exchanged NaMT in order to conclude the reason for a shifted fluorescence peak. Possible causes could be an exchange between Rhodamine 6G and the exchanged clay, thus causing a shift in the wavelength of maximum absorbance. Another possible explanation could be that the exchanged 110% NaMT fluoresces on its own. | *Further tests will need to be done concerning 110% exchanged NaMT in order to conclude the reason for a shifted fluorescence peak. Possible causes could be an exchange between Rhodamine 6G and the exchanged clay, thus causing a shift in the wavelength of maximum absorbance. Another possible explanation could be that the exchanged 110% NaMT fluoresces on its own. |
Revision as of 19:51, 6 March 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
Notes
FluorescenceRhodamine 6G limit of detection:
(1μM)(5000μL)/(92μM)=54.34μL
(0.5μM)(5000μL)/(92μM)=27.17μL
(0.25μM)(5000μL)/(0.5μM)=2.5mL
General information on the parameters of the fluorescence run:
Hydrogel Fluorescence:
General Protocol:
Spectra Obtained:
General Observations
|