User:Moira M. Esson/Notebook/CHEM-581/2013/02/13: Difference between revisions
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* The microspheres prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/08|2013/02/08]] did not form microsphere structures. After placing on the lyophilizer for over 48 hours, a large, solid clump of pure white material formed. This indicates that the emulsion that was prepared was not sufficient for the precipitaiton of microsphere structures. A mortar and pestle was used to grind the large PVOH and clay solid in an attempt to create microsphere structures. DSC will be run of these structures. | * The microspheres prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/08|2013/02/08]] did not form microsphere structures. After placing on the lyophilizer for over 48 hours, a large, solid clump of pure white material formed. This indicates that the emulsion that was prepared was not sufficient for the precipitaiton of microsphere structures. A mortar and pestle was used to grind the large PVOH and clay solid in an attempt to create microsphere structures. DSC will be run of these structures. | ||
* The two hydrogels prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/08|2013/02/08]] were removed from the freezer to thaw for their last freeze-thaw cycle. During the next lab session, these hydrogels will be placed in distilled H<sub>2</sub>O to soak. | * The two hydrogels prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/08|2013/02/08]] were removed from the freezer to thaw for their last freeze-thaw cycle. During the next lab session, these hydrogels will be placed in distilled H<sub>2</sub>O to soak. | ||
* After observation of the hydrogels that were allowed to soak in Rhodamine 6G 1μM solution, it was apparent that the concentration of Rhodamine 6G was enough in the gel. A small, second fraction of 3mL of 1μM Rhodamine 6G was added to the hydrogels. The hydrogels will be allowed to soak until 02/15/13, when diffusion tests will be run on the samples. | * After observation of the hydrogels that were allowed to soak in Rhodamine 6G 1μM solution, it was apparent that the concentration of Rhodamine 6G was enough in the gel. A small, second fraction of 3mL of 1μM Rhodamine 6G was added to the hydrogels. The hydrogels will be allowed to soak until 02/15/13, when diffusion tests will be run on the samples. | ||
* Due to the lack of success with the preparation of PVOH/clay microspheres, a new method for the preparation of microspheres will be used. An aqueous solution of PVOH/clay will be suspended in mineral oil with the necessary amount of DMSO additive. This will be suspended and emulsified for approximately 20 minutes at 90°C. The prepared microspheres will then undergo the freeze thaw method utilized by the hydrogels. | |||
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==Fluorescence== | |||
Revision as of 08:39, 15 February 2013
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