User:Moira M. Esson/Notebook/CHEM-581/2013/02/13: Difference between revisions
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(0.25μM)(5000μL)/(0.5μM)=2.5mL | (0.25μM)(5000μL)/(0.5μM)=2.5mL | ||
*This solution produced a readable spectra. 0.25μM appears to be the upper limit of detection for Rhodamine 6G. All future data will be collected using a 0.25μM concentration of Rhodamine 6G. | *This solution produced a readable spectra. 0.25μM appears to be the upper limit of detection for Rhodamine 6G. All future data will be collected using a 0.25μM concentration of Rhodamine 6G. | ||
General information on the parameters of the fluorescence run: | |||
#Starting wavelength: 500nm | |||
#End wavelength: 650nm | |||
#Excitation wavelength: 480nm | |||
#Exitation slit: 10 | |||
#Emission slit: 10 | |||
#Scan rate/Speed: 1200 | |||
'''Hydrogel Fluorescence''': | '''Hydrogel Fluorescence''': | ||
*Fluorescence was run on the distilled H<sub>2</sub>O that was soaking the hydrogels placed in distilled H<sub>2</sub>O on[[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/06|2013/02/06]] in order to remove any excess DMSO. The hydrogels were soaking in distilled H<sub>2</sub>O for one week. Due to the physical and chemical crosslinking that took place during the 3 cycle freeze-thaw method, it was hoped that all Rhodamine 6G incorporated into the hydrogels through the addition of DMSO/Rhodamine 6G solution would remain in the hydrogel. Fluorescence was run on the samples to determine the concentration of Rhodamine 6G still present in the hydrogels and the rate of diffusion of Rhodamine 6G. | *Fluorescence was run on the distilled H<sub>2</sub>O that was soaking the hydrogels placed in distilled H<sub>2</sub>O on[[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/06|2013/02/06]] in order to remove any excess DMSO. The hydrogels were soaking in distilled H<sub>2</sub>O for one week. Due to the physical and chemical crosslinking that took place during the 3 cycle freeze-thaw method, it was hoped that all Rhodamine 6G incorporated into the hydrogels through the addition of DMSO/Rhodamine 6G solution would remain in the hydrogel. Fluorescence was run on the samples to determine the concentration of Rhodamine 6G still present in the hydrogels and the rate of diffusion of Rhodamine 6G. | ||
General Protocol: | General Protocol: | ||
#Using a transfer pipette, | # Using a transfer pipette, a clean fluorescence cuvette was filled 3/4 of the way full. | ||
# Data obtained was collected between 500-650nm. The excitation wavelength was at 480nm. The scan speed was 1200. | |||
# A fluorescence spectra was obtained. The sample was discarded in the hazardous waste, and any excess Rhodamine 6G containing H<sub>2</sub>O still present in the beaker with the hydrogel was removed. | |||
# Hydrogels were pat dry and then replaced in a beaker. | |||
Spectra Obtained: | |||
<br> | |||
==General Observations== | |||
*Further tests will need to be done concerning 110% exchanged NaMT in order to conclude the reason for a shifted fluorescence peak. Possible causes could be an exchange between Rhodamine 6G and the exchanged clay, thus causing a shift in the wavelength of maximum absorbance. Another possible explanation could be that the exchanged 110% NaMT fluoresces on its own. | |||
Revision as of 15:39, 17 February 2013
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Objectives
Notes
FluorescenceRhodamine 6G limit of detection:
(1μM)(5000μL)/(92μM)=54.34μL
(0.5μM)(5000μL)/(92μM)=27.17μL
(0.25μM)(5000μL)/(0.5μM)=2.5mL
General information on the parameters of the fluorescence run:
Hydrogel Fluorescence:
General Protocol:
Spectra Obtained:
General Observations
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