User:Moira M. Esson/Notebook/CHEM-581/2013/02/08: Difference between revisions
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'''Calculations''' | '''Calculations''' | ||
M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | ||
V<sub>1</sub>=( | V<sub>1</sub>=(1μMx25000μL)/(92μM)=271.74μL | ||
* | *The use of 25mL will make it so a dilution is not necessary for each microsphere dye soak. The prepared 1μM solution will be prepared in a volumetric flask that will be parafilmed and stored for subsequent use. | ||
Revision as of 09:23, 8 February 2013
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Objectives
Dye solution preparation
Calculations M1V1=M2V2 V1=(1μMx25000μL)/(92μM)=271.74μL
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