User:Moira M. Esson/Notebook/CHEM-581/2013/02/08: Difference between revisions
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# Synthesize more PVA-clay microspheres. | # Synthesize more PVA-clay microspheres. | ||
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==Dye | ==Dye solution preparation== | ||
*As with the preparation of the PVA-clay hydrogels, Rhodamine 6G will be used as the dye. An internal concentration of 1μM dye will be used. | *As with the preparation of the PVA-clay hydrogels, Rhodamine 6G will be used as the dye. An internal concentration of 1μM dye will be used. | ||
**Because of the removal of all liquid from the microspheres, a 1μM stock solution will be prepared. A serial dilution will be used from the previously prepared stock solutions. After allowing the the microspheres to absorb the dye for an extended period of time, the microspheres will be rinsed with hot and cold water to remove any DMSO. | **Because of the removal of all liquid from the microspheres, a 1μM stock solution will be prepared. A serial dilution will be used from the previously prepared stock solutions. After allowing the the microspheres to absorb the dye for an extended period of time, the microspheres will be rinsed with hot and cold water to remove any DMSO. |
Revision as of 09:21, 8 February 2013
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Objectives
Dye solution preparation
Calculations M1V1=M2V2 V1=(1μMx2000μL)/(92μM)=21.74μL
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