User:Moira M. Esson/Notebook/CHEM-581/2013/02/06: Difference between revisions
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==PVA Microspheres== | ==PVA Microspheres== | ||
'''General Protocol''': | '''General Protocol''': | ||
*Reaction should be carried out using a reflux condenser and a thermostatic water bath of | *Reaction should be carried out using a reflux condenser and a thermostatic water bath of 55°C. | ||
# Dissolve 1g total of PVA and clay additive in 5mL hot,deionized water. | # Dissolve 1g total of PVA and clay additive in 5mL hot,deionized water. | ||
# Acidify the solution using 0.25mL 0.5M H<sub>2</sub>SO<sub>4</sub>. | # Acidify the solution using 0.25mL 0.5M H<sub>2</sub>SO<sub>4</sub>. | ||
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# Stir the water/1,2-dichlorethane solution for 30 minutes. | # Stir the water/1,2-dichlorethane solution for 30 minutes. | ||
# After 30 minutes stirring, add 0.75mL glutaraldehyde to the solution. | # After 30 minutes stirring, add 0.75mL glutaraldehyde to the solution. | ||
# After the addition of glutaraldehyde, allow the reaction to carry out for 3 additional hours at | # After the addition of glutaraldehyde, allow the reaction to carry out for 3 additional hours at 55°C. | ||
# Filter the produced microspheres from the solution using a a sintered glass filter and a vacuum. | # Filter the produced microspheres from the solution using a a sintered glass filter and a vacuum. | ||
# Wash the collected microspheres using the following solvents in the following order: | # Wash the collected microspheres using the following solvents in the following order: |
Revision as of 08:35, 6 February 2013
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Objectives
PVA MicrospheresGeneral Protocol:
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