User:Moira M. Esson/Notebook/CHEM-581/2013/02/06: Difference between revisions

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==Entry title==
==Objectives==
* Insert content here...
* Prepare PVA(MW 130,000) microspheres through suspension cross-linking of a polymeric solution with glutaraldehyde.
* Remove prepared MW 130,000 PVA hydrogels from freezer and begin soaking in water.
==PVA Microspheres==
'''General Protocol''':
*Reaction should be carried out using a reflux condenser and a thermostatic water bath of 50°C.
# Dissolve 1g PVA in 10mL hot,deionized water.
# Acidify the solution using 0.25mL 0.5M H<sub>2</sub>SO<sub>4</sub>.
# Dissolve 0.6g Cellulose acetate butyrate in 25mL 1,2-dichloroethane.
# Pour the acidified PVA solution into the 25mL 1,2-dichloroethane solution.
# Stir the water/1,2-dichlorethane solution for 30 minutes.
# After 30 minutes stirring, add 0.75mL glutaraldehyde to the solution.
# After the addition of glutaraldehyde, allow the reaction to carry out for 3 additional hours at 50°C.
# Filter the produced microspheres from the solution using a a sintered glass filter and a vacuum.
# Wash the collected microspheres using the following solvents in the following order:
##1,2-dichloroethane
##acetone
##hot water
##cold water
##methanol
#Dry the microspheres at 60°C overnight.
*The following procedure was adapted from the article Poly(vinyl alcohol) microspheres with pH- and thermosensitive properties as temperature-controlled drug delivery [http://www.sciencedirect.com/science/article/pii/S1742706110002151]
 
 
 




Revision as of 17:47, 5 February 2013

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Objectives

  • Prepare PVA(MW 130,000) microspheres through suspension cross-linking of a polymeric solution with glutaraldehyde.
  • Remove prepared MW 130,000 PVA hydrogels from freezer and begin soaking in water.

PVA Microspheres

General Protocol:

  • Reaction should be carried out using a reflux condenser and a thermostatic water bath of 50°C.
  1. Dissolve 1g PVA in 10mL hot,deionized water.
  2. Acidify the solution using 0.25mL 0.5M H2SO4.
  3. Dissolve 0.6g Cellulose acetate butyrate in 25mL 1,2-dichloroethane.
  4. Pour the acidified PVA solution into the 25mL 1,2-dichloroethane solution.
  5. Stir the water/1,2-dichlorethane solution for 30 minutes.
  6. After 30 minutes stirring, add 0.75mL glutaraldehyde to the solution.
  7. After the addition of glutaraldehyde, allow the reaction to carry out for 3 additional hours at 50°C.
  8. Filter the produced microspheres from the solution using a a sintered glass filter and a vacuum.
  9. Wash the collected microspheres using the following solvents in the following order:
    1. 1,2-dichloroethane
    2. acetone
    3. hot water
    4. cold water
    5. methanol
  10. Dry the microspheres at 60°C overnight.
  • The following procedure was adapted from the article Poly(vinyl alcohol) microspheres with pH- and thermosensitive properties as temperature-controlled drug delivery [1]




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