User:Moira M. Esson/Notebook/CHEM-581/2013/02/06

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==Entry title==
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==Objectives==
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* Insert content here...
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* Prepare PVA(MW 130,000) microspheres through suspension cross-linking of a polymeric solution with glutaraldehyde.
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* Remove prepared MW 130,000 PVA hydrogels from freezer and begin soaking in water.
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==PVA Microspheres==
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'''General Protocol''':
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*Reaction should be carried out using a reflux condenser and a thermostatic water bath of 50°C.
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# Dissolve 1g PVA in 10mL hot,deionized water.
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# Acidify the solution using 0.25mL 0.5M H<sub>2</sub>SO<sub>4</sub>.
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# Dissolve 0.6g Cellulose acetate butyrate in 25mL 1,2-dichloroethane.
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# Pour the acidified PVA solution into the 25mL 1,2-dichloroethane solution.
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# Stir the water/1,2-dichlorethane solution for 30 minutes.
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# After 30 minutes stirring, add 0.75mL glutaraldehyde to the solution.
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# After the addition of glutaraldehyde, allow the reaction to carry out for 3 additional hours at 50°C.
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# Filter the produced microspheres from the solution using a a sintered glass filter and a vacuum.
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# Wash the collected microspheres using the following solvents in the following order:
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##1,2-dichloroethane
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##acetone
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##hot water
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##cold water
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##methanol
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#Dry the microspheres at 60°C overnight.
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*The following procedure was adapted from the article Poly(vinyl alcohol) microspheres with pH- and thermosensitive properties as temperature-controlled drug delivery [http://www.sciencedirect.com/science/article/pii/S1742706110002151]
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Revision as of 19:47, 5 February 2013

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Objectives

  • Prepare PVA(MW 130,000) microspheres through suspension cross-linking of a polymeric solution with glutaraldehyde.
  • Remove prepared MW 130,000 PVA hydrogels from freezer and begin soaking in water.

PVA Microspheres

General Protocol:

  • Reaction should be carried out using a reflux condenser and a thermostatic water bath of 50°C.
  1. Dissolve 1g PVA in 10mL hot,deionized water.
  2. Acidify the solution using 0.25mL 0.5M H2SO4.
  3. Dissolve 0.6g Cellulose acetate butyrate in 25mL 1,2-dichloroethane.
  4. Pour the acidified PVA solution into the 25mL 1,2-dichloroethane solution.
  5. Stir the water/1,2-dichlorethane solution for 30 minutes.
  6. After 30 minutes stirring, add 0.75mL glutaraldehyde to the solution.
  7. After the addition of glutaraldehyde, allow the reaction to carry out for 3 additional hours at 50°C.
  8. Filter the produced microspheres from the solution using a a sintered glass filter and a vacuum.
  9. Wash the collected microspheres using the following solvents in the following order:
    1. 1,2-dichloroethane
    2. acetone
    3. hot water
    4. cold water
    5. methanol
  10. Dry the microspheres at 60°C overnight.
  • The following procedure was adapted from the article Poly(vinyl alcohol) microspheres with pH- and thermosensitive properties as temperature-controlled drug delivery [1]




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