User:Moira M. Esson/Notebook/CHEM-581/2013/02/06: Difference between revisions
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== | ==Objectives== | ||
* | * Prepare PVA(MW 130,000) microspheres through suspension cross-linking of a polymeric solution with glutaraldehyde. | ||
* Remove prepared MW 130,000 PVA hydrogels from freezer and begin soaking in water. | |||
==PVA Microspheres== | |||
'''General Protocol''': | |||
*Reaction should be carried out using a reflux condenser and a thermostatic water bath of 50°C. | |||
# Dissolve 1g PVA in 10mL hot,deionized water. | |||
# Acidify the solution using 0.25mL 0.5M H<sub>2</sub>SO<sub>4</sub>. | |||
# Dissolve 0.6g Cellulose acetate butyrate in 25mL 1,2-dichloroethane. | |||
# Pour the acidified PVA solution into the 25mL 1,2-dichloroethane solution. | |||
# Stir the water/1,2-dichlorethane solution for 30 minutes. | |||
# After 30 minutes stirring, add 0.75mL glutaraldehyde to the solution. | |||
# After the addition of glutaraldehyde, allow the reaction to carry out for 3 additional hours at 50°C. | |||
# Filter the produced microspheres from the solution using a a sintered glass filter and a vacuum. | |||
# Wash the collected microspheres using the following solvents in the following order: | |||
##1,2-dichloroethane | |||
##acetone | |||
##hot water | |||
##cold water | |||
##methanol | |||
#Dry the microspheres at 60°C overnight. | |||
*The following procedure was adapted from the article Poly(vinyl alcohol) microspheres with pH- and thermosensitive properties as temperature-controlled drug delivery [http://www.sciencedirect.com/science/article/pii/S1742706110002151] | |||
Revision as of 17:47, 5 February 2013
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Objectives
PVA MicrospheresGeneral Protocol:
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