User:Moira M. Esson/Notebook/CHEM-581/2013/02/06: Difference between revisions
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# Dissolve 1g total of PVA and clay additive in 5mL hot,deionized water. | # Dissolve 1g total of PVA and clay additive in 5mL hot,deionized water. | ||
# Acidify the solution using 0.25mL 0.5M H<sub>2</sub>SO<sub>4</sub>. | # Acidify the solution using 0.25mL 0.5M H<sub>2</sub>SO<sub>4</sub>. | ||
# Dissolve 0.6g Cellulose acetate butyrate in 25mL 1,2-dichloroethane. | # Dissolve 0.6g Cellulose acetate butyrate(CAB) in 25mL 1,2-dichloroethane. | ||
# Pour the acidified PVA solution into the 25mL 1,2-dichloroethane solution. | # Pour the acidified PVA solution into the 25mL 1,2-dichloroethane solution. Place all in a clean round bottom(RB) flask. | ||
# Stir the water/1,2-dichlorethane solution for 30 minutes. | # Stir the water/1,2-dichlorethane solution for 30 minutes. | ||
# After 30 minutes stirring, add 0.75mL glutaraldehyde to the solution. | # After 30 minutes stirring, add 0.75mL glutaraldehyde(GA) to the solution. | ||
# After the addition of glutaraldehyde, allow the reaction to carry out for 3 additional hours at 55°C. | # After the addition of glutaraldehyde, allow the reaction to carry out for 3 additional hours at 55°C. | ||
# Filter the produced microspheres from the solution using a a sintered glass filter and a vacuum. | # Filter the produced microspheres from the solution using a a sintered glass filter and a vacuum. |
Revision as of 11:37, 6 February 2013
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Objectives
PVA MicrospheresGeneral Protocol:
Observations:
Hydrogel Preparation
Clay Filtration |