User:Moira M. Esson/Notebook/CHEM-581/2013/02/01

(Difference between revisions)

Revision as of 15:00, 7 February 2013

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The following hydrogels were prepared following the general protocol described on 2013/01/30. However, the necessary amount of dye was added to each sample prior to freezing using the prepared DMSO/dye stock solution.

Prepared Clay-PVA(MW 130,000) hydrogels	'	'	'	'
Composition of hydrogel(ratio of PVA to clay)	Amount of PVA added(g)	Amount of clay added(g)	Concencentration of DMSO/dye stock solution added(μM)	Amount of DMSO/dye added(mL)
90:10 PVA:50% Bu₃HdP-NaMT	0.92966	0.0999	92	0.0655
50:50 PVA:Bu₃HdP-NaMT	0.5061	0.5001	165	0.0364
90:10 PVA:Lamponite	0.9161	0.1004	92	0.0655
50:50 PVA:Lamponite	0.499	0.5009	165	0.0364
90:10 PVA:NaMT	0.8991	0.1002	92	0.0655
50:50 PVA:NaMT	0.5007	0.4996	165	0.0364
90:10 PVA:110% Lamponite	0.9008	0.0999	92	0.0655
50:50 PVA:110% Lamponite	0.4998		165	0.0364
90:10 PVA:110% NaMT	0.8997	0.1005	92	0.0655

Observations

In order to have a complete and even spread of dye, a glass stirring rod was used to mix the hydrogels.
Before addition of DMSO/dye, the hydrogels were allowed to cool slightly.
Hydrogels with NaMT added did not have as even of a distribution of dye. Upon addition, the dye seemed to sit on top of the hydrogel. Extra stirring was required for these hydrogels.

Surfactant clay exchanges were performed to create a 50% exchanged clay between sodium montmorillonite(NaMT) and Bu₃HdP and 110% exchange between NaMT and Lamponite.
The general protocol for the preparation of 50% exchanged NaMT described on 2012/09/12 was followed.
For the preparation of 110% exchanged NaMT and 110% exchanged Lamponite please refer to Karlena Brown's lab notebook.

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 *Hydrogels with NaMT added did not have as even of a distribution of dye. Upon addition, the dye seemed to sit on top of the hydrogel. Extra stirring was required for these hydrogels.
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 ==Clay Preparation==
 *Surfactant clay exchanges were performed to create a 50% exchanged clay between sodium montmorillonite(NaMT) and Bu<sub>3</sub>HdP and 110% exchange between NaMT and Lamponite.