User:Moira M. Esson/Notebook/CHEM-581/2013/01/30: Difference between revisions
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'''Observations''': | '''Observations''': | ||
*Hydrogels containing sodium montmorillonite(NaMT) required the addition of 1mL distilled H<sub>2</sub>O and required longer heating time. This should be noted when dye is added. A separate, slightly more concentrated stock solution of dye should be prepared for addition to any hydrogels with NaMT additive. | *Hydrogels containing sodium montmorillonite(NaMT) required the addition of 1mL distilled H<sub>2</sub>O and required longer heating time. This should be noted when dye is added. A separate, slightly more concentrated stock solution of dye should be prepared for addition to any hydrogels with NaMT additive. | ||
*After preparation of 6 hydrogels, realized that the inclusion of dye will be necessary before the freeze-thaw | *After preparation of 6 hydrogels, realized that the inclusion of dye will be necessary before the freeze-thaw cross-linking method is performed. I.e. Dye should be added during heating process of PVOH-clay reaction mixture. | ||
*All prepared hydrogels will be reprepared with the addition of dye. | |||
==Calcualtions for dye preparation== | ==Calcualtions for dye preparation== |
Revision as of 08:49, 1 February 2013
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Objectives
Preparation of HydrogelsGeneral Protocol:
The following procedure was adapted from PVA–clay nanocomposite hydrogels for wound dressing
Observations:
Calcualtions for dye preparation
90:10 ratio (1μMx6mL H2O)/0.0655mL DMSO=91.6μM
(90μM dye wanted) x (1x10-6M/1μM) x (0.05L) x (479.02gdye/mol)=0.0022g dye added to 50mL volumetric flask
(1μMx6mL H2O)/0.0364mL DMSO= 164.84μM
164.84μMdye x (1x10-6M/1μM) x (0.05L) x (7479.02g dye/mol)= 0.004g dye added to 50mL volumetric flask Notes
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