User:Moira M. Esson/Notebook/CHEM-581/2013/01/30: Difference between revisions
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*This is the molar concentration of the dye that will need to be prepared. | *This is the molar concentration of the dye that will need to be prepared. | ||
164.84μMdye x (1x10<sup>-6</sup>M/1μM) x (0.05L) x (7479.02g dye/mol)= 0.004g dye added to 50mL volumetric flask | 164.84μMdye x (1x10<sup>-6</sup>M/1μM) x (0.05L) x (7479.02g dye/mol)= 0.004g dye added to 50mL volumetric flask | ||
<br> | |||
'''Rhodamine 6G safety''': | |||
# Rhodamine 6G is mildly flammable. Care must be used at all times when dealing with 1,2-dichloroethane. | |||
# Rhodamine 6G is irritating to eyes. Goggles should be worn whenever using Rhodamine 6G. It is also a fine powder, so open away from face. | |||
# There is limited evidence of the carginogenic effects of Rhodamine 6G. Avoid all Rhodamine 6G skin contact by wearing gloves and goggles. | |||
==Notes== | ==Notes== | ||
*Please refer to Karlena Brown's notebook for protocol for stock solution dye preparation. | *Please refer to Karlena Brown's notebook for protocol for stock solution dye preparation. | ||
Revision as of 07:50, 2 April 2014
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Objectives
Preparation of HydrogelsGeneral Protocol:
The following procedure was adapted from PVA–clay nanocomposite hydrogels for wound dressing
Observations:
Calcualtions for dye preparation
90:10 ratio (1μMx6mL H2O)/0.0655mL DMSO=91.6μM
(90μM dye wanted) x (1x10-6M/1μM) x (0.05L) x (479.02gdye/mol)=0.0022g dye added to 50mL volumetric flask
(1μMx6mL H2O)/0.0364mL DMSO= 164.84μM
164.84μMdye x (1x10-6M/1μM) x (0.05L) x (7479.02g dye/mol)= 0.004g dye added to 50mL volumetric flask
Notes
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