User:Moira M. Esson/Notebook/CHEM-581/2013/01/30: Difference between revisions
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==Notes== | ==Notes== | ||
*Please refer to Karlena Brown's notebook for protocol for stock solution dye preparation. | *Please refer to Karlena Brown's notebook for protocol for stock solution dye preparation. | ||
Revision as of 11:33, 7 February 2013
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Objectives
Preparation of HydrogelsGeneral Protocol:
The following procedure was adapted from PVA–clay nanocomposite hydrogels for wound dressing
Observations:
Calcualtions for dye preparation
90:10 ratio (1μMx6mL H2O)/0.0655mL DMSO=91.6μM
(90μM dye wanted) x (1x10-6M/1μM) x (0.05L) x (479.02gdye/mol)=0.0022g dye added to 50mL volumetric flask
(1μMx6mL H2O)/0.0364mL DMSO= 164.84μM
164.84μMdye x (1x10-6M/1μM) x (0.05L) x (7479.02g dye/mol)= 0.004g dye added to 50mL volumetric flask Notes
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