User:Moira M. Esson/Notebook/CHEM-581/2013/01/30: Difference between revisions
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== | ==Objectives== | ||
* | * Prepare all polyvinyl alcohol(MW 146,000-186,000) hydrogels with clay additives | ||
* Run X-ray diffraction, DSC, and FTIR spectroscopy of all clay samples that were added to the PVOH hydrogels | |||
* Preparation of dye stock solutions. | |||
<br> | |||
==Preparation of Hydrogels== | |||
'''General Protocol''': | |||
# Using a clean 10mL-20mL beaker, add the desired amount of polyvinyl alcohol. (If preparing a 90:10 ratio of polyvinyl alcohol to clay hydrogel, add ~0.9g PVOH. If preparing a 50:50 ratio of polyvinyl alcohol to clay, ~0.5g polyvinyl alcohol should be added. All hydrogels were prepared on a 1g scale.) | |||
# Add the desired amount of clay to the sample. | |||
# Add a magnetic stir bar to the beaker and 6mL of distilled to the reaction mixture. | |||
# Add 8wt% DMSO to the reaction mixture. | |||
# Heat the reaction mixture to 80°C. If the reaction mixture begins to boil, immediately reduce heat. | |||
# Allow the reaction mixture to stir at 80°C for at least 2 hours, or until complete dissolution of polyvinyl alcohol and equal distribution of clay throughout the reaction mixture. | |||
# Remove reaction mixture from heat and place in freezer(-20°C) for 24 hours. After 24 hours, remove from the freezer and allow to thaw for 24 hours. | |||
# Repeat this process three times. | |||
The following procedure was adapted from [http://www.sciencedirect.com/science/article/pii/S0014305706004265 PVA–clay nanocomposite hydrogels for wound dressing] | |||
<br> | |||
'''Hydrogel information''': | |||
*Need to add table of info | |||
'''Observations''': | |||
*Hydrogels containing sodium montmorillonite(NaMT) required the addition of 1mL distilled H<sub>2</sub>O and required longer heating time. This should be noted when dye is added. A separate, slightly more concentrated stock solution of dye should be prepared for addition to any hydrogels with NaMT additive. | |||
*After preparation of 6 hydrogels, realized that the inclusion of dye will be necessary before the freeze-thaw crosslinking method is performed. I.e. Dye should be added during heating process of PVOH-clay reaction mixture. | |||
==Calcualtions for dye preparation== | |||
Revision as of 09:20, 31 January 2013
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Objectives
Preparation of HydrogelsGeneral Protocol:
The following procedure was adapted from PVA–clay nanocomposite hydrogels for wound dressing
Observations:
Calcualtions for dye preparation |