User:Moira M. Esson/Notebook/CHEM-581/2013/01/30: Difference between revisions
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|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Objectives== | ||
* | * Prepare all polyvinyl alcohol(MW 146,000-186,000) hydrogels with clay additives | ||
* Preparation of dye stock solutions. | |||
<br> | |||
==Preparation of Hydrogels== | |||
'''General Protocol''': | |||
# Using a clean 10mL-20mL beaker, add the desired amount of polyvinyl alcohol. (If preparing a 90:10 ratio of polyvinyl alcohol to clay hydrogel, add ~0.9g PVOH. If preparing a 50:50 ratio of polyvinyl alcohol to clay, ~0.5g polyvinyl alcohol should be added. All hydrogels were prepared on a 1g scale.) | |||
# Add the desired amount of clay to the sample. | |||
# Add a magnetic stir bar to the beaker and 6mL of distilled to the reaction mixture. | |||
# Add 8wt% DMSO to the reaction mixture. | |||
# Heat the reaction mixture to 80°C. If the reaction mixture begins to boil, immediately reduce heat. | |||
# Allow the reaction mixture to stir at 80°C for at least 2 hours, or until complete dissolution of polyvinyl alcohol and equal distribution of clay throughout the reaction mixture. | |||
# Remove reaction mixture from heat and place in freezer(-20°C) for 24 hours. After 24 hours, remove from the freezer and allow to thaw for 24 hours. | |||
# Repeat this process three times. | |||
The following procedure was adapted from [http://www.sciencedirect.com/science/article/pii/S0014305706004265 PVA–clay nanocomposite hydrogels for wound dressing] | |||
<br> | |||
'''Hydrogel information''': | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Preparation of PVOH(MW 146,000-186,000)-clay hydrogels''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
|''' Composition of hydrogel'''||'''Amount of PVOH(g)'''||'''Amount of clay(g)'''||'''Amount of DMSO added(mL)''' | |||
|- | |||
| 90:10 PVOH: 50% exchanged NaMT||0.9001||0.1001||0.0655 | |||
|- | |||
| 100% PVOH||1.02193||0||0 | |||
|- | |||
| 50:50 PVOH:50% exchanged NaMT||0.5098||0.4997||0.0364 | |||
|- | |||
| 90:10 PVOH:NaMT||0.90698||0.1002||0.0655 | |||
|- | |||
| 50:50 PVOH:NaMT||0.50399||0.4999||0.0364 | |||
|- | |||
| 90:10 PVOH:Lamponite||0.9062||0.1001||0.0655 | |||
|- | |||
| 50:50 PVOH:Lamponite||0.5105||0.4999||0.0364 | |||
|} | |||
'''Observations''': | |||
*Hydrogels containing sodium montmorillonite(NaMT) required the addition of 1mL distilled H<sub>2</sub>O and required longer heating time. This should be noted when dye is added. A separate, slightly more concentrated stock solution of dye should be prepared for addition to any hydrogels with NaMT additive. | |||
*After preparation of 6 hydrogels, realized that the inclusion of dye will be necessary before the freeze-thaw cross-linking method is performed. I.e. Dye should be added during heating process of PVOH-clay reaction mixture. | |||
*All prepared hydrogels will be reprepared with the addition of dye. | |||
==Calcualtions for dye preparation== | |||
*Using Rhodamine 6G as the dye. This is a very bright and fluorescent dye so will want an internal concentration of dye of 1μM. | |||
'''90:10 ratio''' | |||
(1μMx6mL H<sub>2</sub>O)/0.0655mL DMSO=91.6μM | |||
*This is the molar concentration of the dye will need to be prepared. | |||
(90μM dye wanted) x (1x10<sup>-6</sup>M/1μM) x (0.05L) x (479.02gdye/mol)=0.0022g dye added to 50mL volumetric flask | |||
<br> | |||
'''50:50 ratio''' | |||
(1μMx6mL H<sub>2</sub>O)/0.0364mL DMSO= 164.84μM | |||
*This is the molar concentration of the dye that will need to be prepared. | |||
164.84μMdye x (1x10<sup>-6</sup>M/1μM) x (0.05L) x (7479.02g dye/mol)= 0.004g dye added to 50mL volumetric flask | |||
<br> | |||
'''Rhodamine 6G safety''': | |||
# Rhodamine 6G is mildly flammable. Care must be used at all times when dealing with 1,2-dichloroethane. | |||
# Rhodamine 6G is irritating to eyes. Goggles should be worn whenever using Rhodamine 6G. It is also a fine powder, so open away from face. | |||
# There is limited evidence of the carginogenic effects of Rhodamine 6G. Avoid all Rhodamine 6G skin contact by wearing gloves and goggles. | |||
==Notes== | |||
*Please refer to Karlena Brown's notebook for protocol for stock solution dye preparation. | |||
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Latest revision as of 22:24, 26 September 2017
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Objectives
Preparation of HydrogelsGeneral Protocol:
The following procedure was adapted from PVA–clay nanocomposite hydrogels for wound dressing
Observations:
Calcualtions for dye preparation
90:10 ratio (1μMx6mL H2O)/0.0655mL DMSO=91.6μM
(90μM dye wanted) x (1x10-6M/1μM) x (0.05L) x (479.02gdye/mol)=0.0022g dye added to 50mL volumetric flask
(1μMx6mL H2O)/0.0364mL DMSO= 164.84μM
164.84μMdye x (1x10-6M/1μM) x (0.05L) x (7479.02g dye/mol)= 0.004g dye added to 50mL volumetric flask
Notes
|