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Objectives
- Standardize sodium bicarbonate solution
- Run UV-vis on porphyrin films
- Test swelling on all crosslinked glutaraldehyde films
UV-vis porhyrin films
General protocol for UV-vis on porphyrin films:
- Remove crosslinked porphyrin films from deionized H2O and pat dry using a paper towel
- Using a quartz cuvette as a guide, cut a strip of paper the same width as the quartz cuvette.
- Using the strip of paper as a guide and scissors(or a similar sharp instrument) carefully cut the porphyrin film the same width as the paper reference. When cutting the film, try and use a smooth portion of film.
- Measure the thickness of the film. This will be used as the pathlength.
- Using forceps, carefully place the cut strip of film in a clean quartz cuvette.
- Using a spatula, gently press the film against the cuvette.
- Carefully place the cuvette into the UV-vis.
- Measure following the general protocol for
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