User:Moira M. Esson/Notebook/CHEM-581/2012/11/16

(Difference between revisions)

Revision as of 18:55, 26 November 2012

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General protocol for UV-vis on porphyrin films:

Remove crosslinked porphyrin films from deionized H₂O and pat dry using a paper towel
Using a quartz cuvette as a guide, cut a strip of paper the same width as the quartz cuvette.
Using the strip of paper as a guide and scissors(or a similar sharp instrument) carefully cut the porphyrin film the same width as the paper reference. When cutting the film, try and use a smooth portion of film.
Measure the thickness of the film. This will be used as the pathlength.
Using forceps, carefully place the cut strip of film in a clean quartz cuvette.
Using a spatula, gently press the film against the cuvette.
Carefully place the cuvette into the UV-vis.
Measure following the general protocol for

@@ Line 11: / Line 11: @@
 #Test swelling on all crosslinked glutaraldehyde films
 ==UV-vis porhyrin films==
+General protocol for UV-vis on porphyrin films:
+#Remove crosslinked porphyrin films from deionized H<sub>2</sub>O and pat dry using a paper towel
+#Using a quartz cuvette as a guide, cut a strip of paper the same width as the quartz cuvette.
+#Using the strip of paper as a guide and scissors(or a similar sharp instrument) carefully cut the porphyrin film the same width as the paper reference. When cutting the film, try and use a smooth portion of film.
+#Measure the thickness of the film. This will be used as the pathlength.
+#Using forceps, carefully place the cut strip of film in a clean quartz cuvette.
+#Using a spatula, gently press the film against the cuvette.
+#Carefully place the cuvette into the UV-vis.
+#Measure following the general protocol for
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