User:Moira M. Esson/Notebook/CHEM-581/2012/11/09: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
(9 intermediate revisions by 2 users not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 8: | Line 8: | ||
==Objectives== | ==Objectives== | ||
#Prepare solutions to run new PVOH based reactions | #Prepare solutions to run new PVOH based reactions | ||
#Perform all calculations necessary for future PVOH reactions | |||
*First PVOH reaction will be the synthesis of polyvinyl acetate | |||
*Second PVOH reaction will be the attachment of 5-(4,6-Dichlorotriazinyl)Aminofluorescein which will be referred to as 5-DTAF | |||
==General Formula for reactions== | ==General Formula for reactions== | ||
Polyvinyl alcohol + [HAc]→Polyvinyl acetate | Polyvinyl alcohol + [HAc]→Polyvinyl acetate | ||
<br> | <br> | ||
==General Protocol for Polyvinyl acetate synthesis== | ==General Protocol for Polyvinyl acetate synthesis== | ||
#For a 1:1 ratio of polyvinyl alcohol OH groups to polyvinyl acetate dissolve ~1g PVOH inH<sub>2</sub>O | #For a 1:1 ratio of polyvinyl alcohol OH groups to polyvinyl acetate dissolve ~1g PVOH inH<sub>2</sub>O | ||
Line 20: | Line 25: | ||
#Rotavap the remaining reaction mixture | #Rotavap the remaining reaction mixture | ||
<br> | <br> | ||
==Calculations== | ==Calculations== | ||
*Overall, want to determine if have 1g of material how many moles of -OH groups. Once that is determined, will want to determine how many moles of -OH was reacted after the reaction. | *Overall, want to determine if have 1g of material how many moles of -OH groups. Once that is determined, will want to determine how many moles of -OH was reacted after the reaction. | ||
Line 36: | Line 42: | ||
<br> | <br> | ||
2.27x10<sup>-6</sup> moles dye x(495.28g dye/1mol dye)=0.001124g dye | 2.27x10<sup>-6</sup> moles dye x(495.28g dye/1mol dye)=0.001124g dye | ||
<br> | |||
'''Polyvinyl acetate synthesis calculations''': | |||
*For all reaction mixtures, the total volume of the reaction mixtures will be 7mL | |||
* HAc will be the abbreviation for acetic acid | |||
<br> | |||
Density of HAc= 1.05g/mL | |||
(A.) 1:1 ratio | |||
1g PVOH x (1mol PVOH/44g PVOH) x (1mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 1.36g HAc | |||
1.36g HAc x (1mL/1.05g HAc)= 1.295mL HAc | |||
(B.) 20% ratio | |||
1gPVOH x (1mol PVOH/44g PVOH) x (0.2mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 0.273g HAc | |||
0.273g HAc x (1mL/1.05g HAc)=0.26mL HAc | |||
(C.) 40% ratio | |||
1gPVOH x (1mol PVOH/44g PVOH) x (0.4mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 0.545g HAc | |||
0.5195g HAc x (1mL/1.05g)= 0.5195mL HAc | |||
(D.) 60% ratio | |||
1gPVOH x (1mol PVOH/44g PVOH) x (0.6mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 0.818g HAc | |||
0.818g HAc x (1mL HAc/ 1.05g)= 0.779mL HAc | |||
<br> | |||
'''Titration calcualtions''': | |||
<br> | |||
(A.) Sodium bicarbonate solution preparation | |||
0.2L x (1mol/1L) x (84.01g/1mol)= 16.802g sodium bicarbonate. | |||
*Note: The actual amount of sodium bicarbonate used was 16.8046g. Also, it is not necessary to prepare this solution in a volumetric flask. Will standardize the solution to know the actual concentration. | |||
<br> | |||
(B.)Potassium hydrogen phthalate(PHP) needed | |||
MW PHP= 204.24g/mol | |||
1M PHP= x/0.1L | |||
x=0.1mol x 204.24g/mol | |||
x= 20.424g PHP needed | |||
==Solution Preparations== | |||
<br> | |||
(A.) Sodium bicarbonate standard | |||
# ~16.802g sodium bicarbonate was measured and placed in a 200mL plastic container. | |||
# 200mL of deionized H<sub>2</sub>O was measured using a graduated cylinder and was poured into the plastic container. | |||
# The plastic container was sealed and the bottle was swirled vigorously until all sodium bicarbonate was dissolved. | |||
<br> | |||
(B.)PHP solution preparation | |||
# 20.424g PHP was measured and placed in a 120°C oven for 1 hour to dry | |||
# Dried PHP was placed in a 200mL volumetric flask with a magnetic stir bar and 100mL of deionized H<sub>2</sub>O was added to the flask | |||
# A rubber stopper was placed on the top of the volumetric flask and the mixture was allowed to stir for 72 hours. | |||
<br> | |||
(C.)Reaction mixture preparations | |||
# ~1g of PVOH was measured and placed in a 10mL beaker | |||
# The necessary amount of deionized H<sub>2</sub>O was added to the beaker(7mL - the amount of HAc that would be added) and a small stir bar was placed in the beaker | |||
# The beaker was parafilmed and set in a drawer. | |||
# The following process was followed for each of the 4 polyvinyl acetate reactions | |||
Actual amount of PVOH measured: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Ratio of polyvinyl acteate and polyvinyl alcohol''' | |||
| align="center" style="background:#f0f0f0;"|'''Amount of PVOH(MW 22K) used (g)''' | |||
|- | |||
| 1 to 1 reaction||1.0062 | |||
|- | |||
| 20%||1.0043 | |||
|- | |||
| 40%||1.0052 | |||
|- | |||
| 60%||1.0027 | |||
|} | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Latest revision as of 22:14, 26 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||
Objectives
General Formula for reactionsPolyvinyl alcohol + [HAc]→Polyvinyl acetate
General Protocol for Polyvinyl acetate synthesis
Calculations
Number of moles of OH groups in 1g PVOH: MW of repeating polyvinyl alcohol unit [C2H3OH]: 44.06g/mol Polyvinyl alcohol repeating unit picture 1gPVOH(1mol PVOH unit/44.06g) x (1mol OH/1mol PVOH unit)= 0.022696 mol OH/g Dye attachment calculations:
MW 5-DTAF dye: 495.28g/mol
(0.0227mol OH/g) x (.0001)= 2.27x10-6 moles dye
2.27x10-6 moles dye x(495.28g dye/1mol dye)=0.001124g dye
1g PVOH x (1mol PVOH/44g PVOH) x (1mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 1.36g HAc 1.36g HAc x (1mL/1.05g HAc)= 1.295mL HAc (B.) 20% ratio 1gPVOH x (1mol PVOH/44g PVOH) x (0.2mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 0.273g HAc 0.273g HAc x (1mL/1.05g HAc)=0.26mL HAc (C.) 40% ratio 1gPVOH x (1mol PVOH/44g PVOH) x (0.4mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 0.545g HAc 0.5195g HAc x (1mL/1.05g)= 0.5195mL HAc (D.) 60% ratio 1gPVOH x (1mol PVOH/44g PVOH) x (0.6mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 0.818g HAc 0.818g HAc x (1mL HAc/ 1.05g)= 0.779mL HAc
0.2L x (1mol/1L) x (84.01g/1mol)= 16.802g sodium bicarbonate.
1M PHP= x/0.1L x=0.1mol x 204.24g/mol x= 20.424g PHP needed Solution Preparations
Actual amount of PVOH measured:
|