User:Moira M. Esson/Notebook/CHEM-581/2012/10/26

Objectives

1. Crosslink porphyrin films
2. Run AA using film adsorption
3. Prepare next highest porphyrin concentration solution. Then add salts to these higher porphyrin solutions

Crosslinking

• The general protocol for porphyrin film crosslinking described on 2012/09/14 was followed for the films listed below

Films Crosslinked:

1. PVOH(22K) 1.0073g+1.0mL GA+1mL porphyrin
2. PVOH(22K) 1.05225g+1.0mL GA+1mL porphyrin

Crosslinking information

• For the films listed below, the films were placed in a 6M NaCl solution at 70°C for ~1.5 hours

Crosslinked Films:

1. PVOH(22K) 1.02114g+0.5mL GA+1mL porphyrin+HCl
2. PVOH(22K) 1.0067g+1mL GA+1mL porphyrin+HCl

Crosslinking information:

Observations for crosslinking films

• PVOH(22K) 1.02114g+0.5mL GA+1mL porphyrin+HCl was shrunken, shriveled and was green in color before crosslinking
• PVOH(22K) 1.0067g+1mL GA+1mL porphyrin+HCl shrunk, looked shriveled and was green in color before crosslinking
• PVOH(22K) 1.0073g+1.0mL GA+1mL porphyrin was flat, relatively shiny and pink in color before crosslinking
• PVOH(22K) 1.05225g+1.0mL GA+1mL porphyrin was flat, shiny and pink in color before crosslinking
• When added film to fixant solution both beakers of solution had some color leak out. Color of both beakers were yellowish/greenish. Film with 1mL GA not as colored. Film with 0.5mL GA seems to be a more vibrant color(leaking more)
• NaCl solution with film with 0.5mL GA had very slight leaking(slight discoloration of solution very light green). Coloration of solution was occurring very gradually
• Both films after fixant solution were a vibrant green color(like jolly rancher green). Film with 1.0mL GA was a darker/ more vibrant green
• Films placed in 1% aqueous HCl soltn. both started to have color leak out
• 1.0mL GA+HCl film when placed in the 6M NaCl solution leaked very minimally(solution looks very faintly green). Much less in comparison to 0.5mL GA.
• 0.5mL GA+HCl film after 1hr. and 15min. heated , remained bright green color. Stored in H₂O. After adding H₂O, film turned into a pink color(darker pink on edges)
• Addition of both films to sodium bicarbonate caused all porphyrin to leak out(films no longer colored. completely clear.)
• Film 1mLGA+HCl remained dark green and more stiff than other film. Turned pink after addition to deionized H₂O

Please refer to User:Karlena_L._Brown/Notebook/CHEM-581/2012/10/26 and User:Melissa_Novy/Notebook/CHEM-581/2012/10/26 for calculations relating to preparation of higher concentration of porphyrin solutions and salt.