User:Moira M. Esson/Notebook/CHEM-571/2014/04/01

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(Atomic Absorption)
(Atomic Absorption)
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==Atomic Absorption==
==Atomic Absorption==
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*The general protocol for AA was followed. Samples were analyzed for the presence of iron.
[[Image:Chem572day4-1.png|500px]]
[[Image:Chem572day4-1.png|500px]]
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<br>
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Table 1. Atomic Absorbance Data for Magnetite:Protein hydrogel samples
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Sample number'''
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| align="center" style="background:#f0f0f0;"|'''Sample name'''
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| align="center" style="background:#f0f0f0;"|'''Abs'''
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| align="center" style="background:#f0f0f0;"|'''background'''
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| align="center" style="background:#f0f0f0;"|'''actual abs.'''
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|-
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| 1||13000:1 mag- BSA+ 130:1 Au-BSA ||0.0117||-0.0005||0.0122
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|-
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| 2||13000:1 mag-BSA  sonicated||0.0029||0.007||-0.0041
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|-
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| 3||13500:1 Mag-BSA + 135:1 Au-BSA||0.0022||-0.0019||0.0041
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|-
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| 4||17000:1 Mag-Heme +170:1 Au-Heme||0.0029||-0.0013||0.0042
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|-
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| 5||16500:1 Mag-Heme +165:1 Au-Heme||0.002||-0.0021||0.0041
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|-
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| 6||16500:1 Mag-Heme sonicator||-0.0009||0.0162||-0.0171
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|-
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| 7||16000:1 Mag-Heme+ 160:1 Au-Heme||0.0023||0.00066||0.00164
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|}
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<br>
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Table 2. Concentration of Iron present in each sample
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Sample name'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Iron in sample (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Total concentration Iron originally in solution (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Iron incorporated (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Percent Iron incorporated (%)'''
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|-
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| 13000:1 mag- BSA+ 130:1 Au-BSA ||-0.01359993||1765.35||1765.3636||100.0007704
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|-
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| 13000:1 mag-BSA  sonicated||-0.01375041||1765.35||1765.36375||100.0007789
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|-
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| 13500:1 Mag-BSA + 135:1 Au-BSA||-0.01376238||1765.35||1765.363762||100.0007796
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|-
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| 17000:1 Mag-Heme +170:1 Au-Heme||-0.01375041||1765.35||1765.36375||100.0007789
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|-
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| 16500:1 Mag-Heme +165:1 Au-Heme||-0.0137658||1765.35||1765.363766||100.0007798
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|-
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| 16500:1 Mag-Heme sonicator||-0.01381539||1765.35||1765.363815||100.0007826
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|-
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| 16000:1 Mag-Heme+ 160:1 Au-Heme||-0.01376067||1765.35||1765.363761||100.0007795
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|}
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Revision as of 08:56, 29 April 2014

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Objectives

  1. Schlenk line method sample prep
  2. Fe atomic absorption of samples prepared on 03/18/2014 and 03/19/2014


Schlenk line sample preparation


Atomic Absorption

  • The general protocol for AA was followed. Samples were analyzed for the presence of iron.


Table 1. Atomic Absorbance Data for Magnetite:Protein hydrogel samples

Sample number Sample name Abs background actual abs.
113000:1 mag- BSA+ 130:1 Au-BSA 0.0117-0.00050.0122
213000:1 mag-BSA sonicated0.00290.007-0.0041
313500:1 Mag-BSA + 135:1 Au-BSA0.0022-0.00190.0041
417000:1 Mag-Heme +170:1 Au-Heme0.0029-0.00130.0042
516500:1 Mag-Heme +165:1 Au-Heme0.002-0.00210.0041
616500:1 Mag-Heme sonicator-0.00090.0162-0.0171
716000:1 Mag-Heme+ 160:1 Au-Heme0.00230.000660.00164


Table 2. Concentration of Iron present in each sample

Sample name Concentration Iron in sample (ug/mL) Total concentration Iron originally in solution (ug/mL) Concentration Iron incorporated (ug/mL) Percent Iron incorporated (%)
13000:1 mag- BSA+ 130:1 Au-BSA -0.013599931765.351765.3636100.0007704
13000:1 mag-BSA sonicated-0.013750411765.351765.36375100.0007789
13500:1 Mag-BSA + 135:1 Au-BSA-0.013762381765.351765.363762100.0007796
17000:1 Mag-Heme +170:1 Au-Heme-0.013750411765.351765.36375100.0007789
16500:1 Mag-Heme +165:1 Au-Heme-0.01376581765.351765.363766100.0007798
16500:1 Mag-Heme sonicator-0.013815391765.351765.363815100.0007826
16000:1 Mag-Heme+ 160:1 Au-Heme-0.013760671765.351765.363761100.0007795


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