User:Moira M. Esson/Notebook/CHEM-571/2014/02/05

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(BSA:Magnetite nanoparticle preparation)
(BSA:Magnetite nanoparticle preparation)
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Table 6. Preparation of KNO<sub>3</sub> stock solution for Reaction Mixture 2
Table 6. Preparation of KNO<sub>3</sub> stock solution for Reaction Mixture 2
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
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| align="center" style="background:#f0f0f0;"|''''''
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| Molecular Weight (g/mol)||101.1
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| mass(g)||0.5604
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| volume stock (L)||0.01
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| Concentration (M)||0.5
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==FTIR and XRD Sample Preparation==  
==FTIR and XRD Sample Preparation==  

Revision as of 21:08, 10 February 2014

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Objective

  1. Prepare all magnetite samples that were synthesized on 01/28/2014 and 01/29/2014 for FTIR and XRD analysis.
  2. Analyze magnetite nanoparticle/protein fiber samples prepared on 02/04/2014 using UV/vis spectroscopy.
  3. Synthesize two different ratios of BSA:Magnetite nanoparticles/fibers


BSA:Magnetite nanoparticle preparation

  • The general protocol described on 01/29/2014 was followed.
  • Two solutions were prepared. Both solutions were prepared as a 13000:1 BSA:Magnetite.
    • The difference between the two solutions is the concentration of KNO3 that was added to the solution.
    • Reaction mixture 1 contains the concentration of KNO3 that follows the protocol adaptation from the following reference [[1]].
    • Reaction mixture 2 contains the concentration of KNO3 described on 02/04/2014.


Table 1. Preparation of both reaction mixtures

Ratio concentration BSA (M) volume of BSA added (mL) amount of FeSO4 added (mL) amount of KOH added (mL) amount of KNO3 added (mL) volume water added (mL)
130002.30769E-060.4592100410.50.5006245542.51.040165405


Table 2. Preparation of BSA stock solution

Preparation of BSA stock '
Molecular weight (g/mol)66463
mass (g)0.0167
volume stock (L)0.01
Concentration (M)2.51268E-05


Table 3. Preparation of KOH stock solution

Preparation of KOH stock '
Molecular Weight (g/mol)56.11
mass(g)0.5604
volume stock (L)0.01
Concentration (M)0.998752451


Table 4. Preparation of FeSO4*7H2O stock solution

Preparation of FeSO4 stock '
Molecular Weight (g/mol)278.01
mass(g)0.83403
volume stock (L)0.01
Concentration (M)0.3


Table 5. Preparation of KNO3 stock solution for Reaction Mixture 1

Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)4.044
volume stock (L)0.05
Concentration (M)0.8


Table 6. Preparation of KNO3 stock solution for Reaction Mixture 2

Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)0.5604
volume stock (L)0.01
Concentration (M)0.5


FTIR and XRD Sample Preparation

Due to the absence of an iron lamp for AA,

UV/vis

Observations

  • The Hemoglobin:magnetite colloid suspension/fibers prepared on 02/04/2014 formed larger particles than the previously prepared solution using the Schlenk line. The resulting reaction mixture was clear other than the formed particles at the bottom of the round bottom flask. Using a magnet, the particles from the reaction mixture prepared on 02/04/2014 appeared to be more magnetic (i.e. were more easily moved when an external magnetic field was applied) than the particles prepared on 01/29/2014. This may be due to the different ratio of protein to FeSO4 used. As such, two new BSA:magnetite solutions were prepared in order to compare.



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