User:Moira M. Esson/Notebook/CHEM-571/2014/02/05

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(Observations)
(FTIR and XRD Sample Preparation)
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*All samples that were centrifuged on [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/04|02/04/2014]]
*All samples that were centrifuged on [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/04|02/04/2014]]
Due to the absence of an iron lamp for AA,
Due to the absence of an iron lamp for AA,
 +
==UV/vis==
 +
==Observations==
==Observations==
*The Hemoglobin:magnetite colloid suspension/fibers prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/04|02/04/2014]] formed larger particles than the previously prepared solution using the Schlenk line. The resulting reaction mixture was clear other than the formed particles at the bottom of the round bottom flask. Using a magnet, the particles from the reaction mixture prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/04|02/04/2014]] appeared to be more magnetic (i.e. were more easily moved when an external magnetic field was applied) than the particles prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/04|01/29/2014]]. This may be due to the different ratio of protein to FeSO<sub>4</sub> used. As such, two new BSA:magnetite solutions were prepared in order to compare.   
*The Hemoglobin:magnetite colloid suspension/fibers prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/04|02/04/2014]] formed larger particles than the previously prepared solution using the Schlenk line. The resulting reaction mixture was clear other than the formed particles at the bottom of the round bottom flask. Using a magnet, the particles from the reaction mixture prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/04|02/04/2014]] appeared to be more magnetic (i.e. were more easily moved when an external magnetic field was applied) than the particles prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/04|01/29/2014]]. This may be due to the different ratio of protein to FeSO<sub>4</sub> used. As such, two new BSA:magnetite solutions were prepared in order to compare.   

Revision as of 20:43, 10 February 2014

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Objective

  1. Prepare all magnetite samples that were synthesized on 01/28/2014 and 01/29/2014 for FTIR and XRD analysis.
  2. Analyze magnetite nanoparticle/protein fiber samples prepared on 02/04/2014 using UV/vis spectroscopy.
  3. Synthesize two different ratios of BSA:Magnetite nanoparticles/fibers


BSA:Magnetite nanoparticle preparation

  • The general protocol described on 01/29/2014 was followed.

FTIR and XRD Sample Preparation

Due to the absence of an iron lamp for AA,

UV/vis

Observations

  • The Hemoglobin:magnetite colloid suspension/fibers prepared on 02/04/2014 formed larger particles than the previously prepared solution using the Schlenk line. The resulting reaction mixture was clear other than the formed particles at the bottom of the round bottom flask. Using a magnet, the particles from the reaction mixture prepared on 02/04/2014 appeared to be more magnetic (i.e. were more easily moved when an external magnetic field was applied) than the particles prepared on 01/29/2014. This may be due to the different ratio of protein to FeSO4 used. As such, two new BSA:magnetite solutions were prepared in order to compare.



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