User:Moira M. Esson/Notebook/CHEM-571/2014/02/04

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(Preparation of Hemoglobin:Magnetite Solution)
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#Prepare a Hemoglobin:Magnetite solution following the general protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/29|01/29/2014]]
#Prepare a Hemoglobin:Magnetite solution following the general protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/29|01/29/2014]]
#Prepare a hemoglobin:Magnetite solution of the same ratio following the general protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]] for comparison
#Prepare a hemoglobin:Magnetite solution of the same ratio following the general protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]] for comparison
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#Prepare a hemoglobin:Au solution of the same ratio using the general method of protein-AuNP preparation described on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]] for future comparison.
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#Prepare BSA:Magnetite solutions of the same ratio using the general method of protein-Au preparation in the oven described on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]].
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#Filter prepared BSA:Au:Magnetite solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]] using a Whatman filter paper with a pore size of 20-25μ. This filtrate will be dried and analyzed using DSC, FTIR and XRD.
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#Centrifuge BSA:Au:Magnetite soluions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]]. This will be used for AA analysis tomorrow. The supernatent will be collected, dried, and analyzed using DSC, FTIR and X-ray.  
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#Centrifuge recollected solution for future AA analysis.
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==Preparation of Hemoglobin:Magnetite Solution==
==Preparation of Hemoglobin:Magnetite Solution==
*The general protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/29|01/29/2014]] was followed.
*The general protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/29|01/29/2014]] was followed.

Revision as of 15:59, 4 February 2014

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Objectives

  1. Prepare a Hemoglobin:Magnetite solution following the general protocol described on 01/29/2014
  2. Prepare a hemoglobin:Magnetite solution of the same ratio following the general protocol described on 01/28/2014 for comparison
  3. Prepare BSA:Magnetite solutions of the same ratio using the general method of protein-Au preparation in the oven described on 01/28/2014.
  4. Centrifuge BSA:Au:Magnetite soluions prepared on 01/28/2014. This will be used for AA analysis tomorrow. The supernatent will be collected, dried, and analyzed using DSC, FTIR and X-ray.


Preparation of Hemoglobin:Magnetite Solution

  • The general protocol described on 01/29/2014 was followed.

Table 1. Preparation of Hemoglobin stock solution

Preparation of hemoglobin Stock solution '
Molecular weight (g/mol)64500
mass hemglobin (g)0.1
volume solution (L)0.1
Concentration (M)1.55039E-05


Table 2. Preparation of FeSO4*7H2O stock solution

Preparation of FeSO4 stock '
Molecular Weight (g/mol)278.01
mass(g)0.83403
volume stock (L)0.01
Concentration (M)0.3


Table 3. Preparation of KOH stock solution

Preparation of KOH stock '
Molecular Weight (g/mol)56.11
mass(g)0.5606
volume stock (L)0.01
Concentration (M)0.999108893


Table 4. Preparation of KNO3 stock solution


Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)4.044
volume stock (L)0.05
Concentration (M)0.8


Table 5. Preparation of Hemoglobin:Magnetite solution

Ratio concentration Hemoglobin (M) volume of Hemoglobin added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
130002.30769E-060.7442307690.50.5004459512.50.75532328


  • The ratio is two orders of magnitude larger than the ratio when gold is used for prep due to the fact this ratio refers to the concentration of FeSO4 in solution (which is 0.03M). As such, the ratio was altered to make similar concentration comparisons (with this order of magnitude it is comparing to 0.003M which is similar to the 0.0025M gold in the previous solutions)



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