User:Moira M. Esson/Notebook/CHEM-571/2014/01/29

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(Objectives)
(Objectives)
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==Objectives==
==Objectives==
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#Prepare a BSA-Magnetite solution for later testing.
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#Prepare a BSA-Magnetite solution with the purpose of synthesizing BSA fibers with magnetite nanoparticles incorporated in the fiber.
#Analyze protein-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]] using UV/vis.
#Analyze protein-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]] using UV/vis.
#Analyze protein-AuNP-Magnetite solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]] using UV/vis.
#Analyze protein-AuNP-Magnetite solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]] using UV/vis.
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==Preparation of BSA-Magentite solution==
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*The following protocol was
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==UV/vis==
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'''General Protocol''':
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# Place 1mL of each ratio in an 1.5mL eppendorf tube.
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# Centrifuge at maximum speed for 30 minutes.
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# Take a UV-Vis spectrum of each sample from 200nm to 800nm.
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# After taking the spectrum, store the sample in a fresh eppy tube in an upright position.
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Figure 1. Corrected Absorbance Spectra of BSA-AuNP solutions (...ratios)
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==Notes==
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Revision as of 20:12, 3 February 2014

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Objectives

  1. Prepare a BSA-Magnetite solution with the purpose of synthesizing BSA fibers with magnetite nanoparticles incorporated in the fiber.
  2. Analyze protein-AuNP solutions prepared on 01/28/2014 using UV/vis.
  3. Analyze protein-AuNP-Magnetite solutions prepared on 01/28/2014 using UV/vis.


Preparation of BSA-Magentite solution

  • The following protocol was

UV/vis

General Protocol:

  1. Place 1mL of each ratio in an 1.5mL eppendorf tube.
  2. Centrifuge at maximum speed for 30 minutes.
  3. Take a UV-Vis spectrum of each sample from 200nm to 800nm.
  4. After taking the spectrum, store the sample in a fresh eppy tube in an upright position.


Figure 1. Corrected Absorbance Spectra of BSA-AuNP solutions (...ratios)

Notes


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