Objectives
- Synthesize new Myoglobin-AuNP solutions with different ratios in order to determine the optimal concentration of Au to protein for the formation of fibers
- Synthesize Lysozyme-AuNP solutions
Myoglobin-AuNP and Lysozyme-AuNP synthesis
- The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on 08/28/2013.
- 5mL of each solution was prepared.
Table 1. Preparation of Myoglobin-AuNP solutions
| Ratio Myoglobin
|
Amount of gold added(mL)
|
Concentration Myoglobin(M)
|
Volume Myoglobin(mL)
|
Volume Water Myoglobin(mL)
|
| 5 |
0.494264558 |
0.00005 |
2 |
2.505735442
|
| 10 |
0.494264558 |
0.000025 |
1 |
3.505735442
|
| 15 |
0.494264558 |
1.66667E-05 |
0.666666667 |
3.839068775
|
| 20 |
0.494264558 |
0.0000125 |
0.5 |
4.005735442
|
| 25 |
0.494264558 |
0.00001 |
0.4 |
4.105735442
|
| 30 |
0.494264558 |
8.33333E-06 |
0.333333333 |
4.172402108
|
| 35 |
0.494264558 |
7.14286E-06 |
0.285714286 |
4.220021156
|
| 40 |
0.494264558 |
0.00000625 |
0.25 |
4.255735442
|
| 45 |
0.494264558 |
5.55556E-06 |
0.222222222 |
4.28351322
|
| 50 |
0.494264558 |
0.000005 |
0.2 |
4.305735442
|
Table 2. Preparation of all Lysozyme-AuNP solutions
| Ratio Lysozyme
|
Amount gold added (mL)
|
Concentration Lysozyme(M)
|
Volume Lysozyme (mL)
|
Volume Water Lysozyme (mL)
|
| 30 |
0.494264558 |
8.33333E-06 |
1.611148649 |
2.894586793
|
| 50 |
0.494264558 |
0.000005 |
0.966689189 |
3.539046253
|
| 70 |
0.494264558 |
3.57143E-06 |
0.690492278 |
3.815243164
|
| 90 |
0.494264558 |
2.77778E-06 |
0.53704955 |
3.968685892
|
| 110 |
0.494264558 |
2.27273E-06 |
0.439404177 |
4.066331265
|
| 130 |
0.494264558 |
1.92308E-06 |
0.371803534 |
4.133931907
|
| 150 |
0.494264558 |
1.66667E-06 |
0.32222973 |
4.183505712
|
| 170 |
0.494264558 |
1.47059E-06 |
0.28432035 |
4.221415092
|
| 190 |
0.494264558 |
1.31579E-06 |
0.254391892 |
4.25134355
|
| 210 |
0.494264558 |
1.19048E-06 |
0.230164093 |
4.275571349
|
Table 3. Stock Solution concentrations
| Concentration of Myoglobin (M)
|
Concentration Gold(M)
|
Concentration Lysozyme (M)
|
| 0.000125 |
0.00252901 |
2.58615E-05
|
- All stock solutions were prepared with deionized H2O
- The gold stock solution was prepared using Gold Chloride (II) trihydrate
Table 4. Preparation of Lysozyme Stock solution
| Molecular Weight(g/mol)
|
14307
|
| mass lysozyme(g) |
0.0037
|
| volume solution(mL) |
10
|
| Concentration Lysozyme (M) |
2.58615E-05
|
Table 5. Preparation of Myoglobin Stock solution
| Molecular weight (g/mol)
|
17600
|
| mass myoglobin (g) |
0.022
|
| volume solution (mL) |
0.01
|
| Concentration (M) |
0.000125
|
Notes
- All of the prepared AuNP solutions had a slight yellow tinge to the solutions (from the presence of the gold).
- All of the prepared AuNP solutions will be tested using UV/vis and AA spectroscopy
| 
Project name
