User:Moira M. Esson/Notebook/CHEM-571/2013/10/23: Difference between revisions
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==UV/vis of Pepsin-AuNP and HRP-AuNP== | ==UV/vis of Pepsin-AuNP and HRP-AuNP== | ||
'''General Protocol''': | |||
# Place 1mL of each ratio in an 1.5mL eppendorf tube. | # Place 1mL of each ratio in an 1.5mL eppendorf tube. | ||
# Centrifuge at maximum speed for 30 minutes. | # Centrifuge at maximum speed for 30 minutes. | ||
# Take a UV-Vis spectrum of each sample from 200nm to 800nm. | # Take a UV-Vis spectrum of each sample from 200nm to 800nm. | ||
# After taking the spectrum, store the sample in a fresh eppy tube in an upright position. | # After taking the spectrum, store the sample in a fresh eppy tube in an upright position. | ||
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Figure 1. Corrected Absorbance spectra of pepsin-AuNPs | |||
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[[Image:Pepsin Nano.png]] | |||
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*Both 30:1 and 70:1 pepsin-AuNP had an absorbance peak at 518nm. | |||
==Notes== | ==Notes== | ||
*The remaining UV/vis samples will be run during the next lab period. | *The remaining UV/vis samples will be run during the next lab period. | ||
*Although | *Although neither the HRP or the pepsin solutions formed fibers, a black residue and color change(see figure 2) was observed for the pepsin solutions. The black residue will be tested in the future for identification. | ||
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Revision as of 14:26, 29 October 2013
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Objectives
HRP-AuNP and Myoglobin-AuNP
UV/vis of Pepsin-AuNP and HRP-AuNPGeneral Protocol:
Notes
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