User:Moira M. Esson/Notebook/CHEM-571/2013/10/23: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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*The protein-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/22|10/22/2013]] did not produce fibers. As such, new protein-AuNP solutions with increased ratios of protein-AuNP were prepared in order to determine the optimal concentration of Au to protein to synthesize fibers. | *The protein-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/22|10/22/2013]] did not produce fibers. As such, new protein-AuNP solutions with increased ratios of protein-AuNP were prepared in order to determine the optimal concentration of Au to protein to synthesize fibers. | ||
*The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]]. | *The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]]. | ||
*5mL of each solution was prepared. | |||
<br> | <br> | ||
Table 1. Stock solutions used in the preparation of Protein-AuNP solutions | Table 1. Stock solutions used in the preparation of Protein-AuNP solutions | ||
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| 0.0000015||0.0025||0.0000165 | | 0.0000015||0.0025||0.0000165 | ||
|} | |} | ||
Table 2. Preparation of | <br> | ||
Table 2. Preparation of HRP-AuNP solutions | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Amount of gold added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration HRP (M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume HRP (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume H<sub>2</sub>O added (mL)''' | |||
|- | |||
| 230||0.5||1.08696E-06||3.623188406||0.876811594 | |||
|- | |||
| 250||0.5||0.000001||3.333333333||1.166666667 | |||
|- | |||
| 270||0.5||9.25926E-07||3.086419753||1.413580247 | |||
|- | |||
| 290||0.5||8.62069E-07||2.873563218||1.626436782 | |||
|- | |||
| 310||0.5||8.06452E-07||2.688172043||1.811827957 | |||
|- | |||
| 330||0.5||7.57576E-07||2.525252525||1.974747475 | |||
|- | |||
| 350||0.5||7.14286E-07||2.380952381||2.119047619 | |||
|- | |||
| 370||0.5||6.75676E-07||2.252252252||2.247747748 | |||
|- | |||
| 390||0.5||6.41026E-07||2.136752137||2.363247863 | |||
|- | |||
| 410||0.5||6.09756E-07||2.032520325||2.467479675 | |||
|} | |||
<br> | |||
Table 3. Preparation of Myoglobin-AuNPs | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Amount of gold added (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration Myoglobin (M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume of myogloblin(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Water Myoglobin(mL)''' | |||
|- | |||
| 30||0.5||8.33333E-06||2.525252525||1.974747475 | |||
|- | |||
| 50||0.5||0.000005||1.515151515||2.984848485 | |||
|- | |||
| 70||0.5||3.57143E-06||1.082251082||3.417748918 | |||
|- | |||
| 90||0.5||2.77778E-06||0.841750842||3.658249158 | |||
|- | |||
| 110||0.5||2.27273E-06||0.688705234||3.811294766 | |||
|- | |||
| 130||0.5||1.92308E-06||0.582750583||3.917249417 | |||
|- | |||
| 150||0.5||1.66667E-06||0.505050505||3.994949495 | |||
|- | |||
| 170||0.5||1.47059E-06||0.445632799||4.054367201 | |||
|- | |||
| 190||0.5||1.31579E-06||0.398724083||4.101275917 | |||
|- | |||
| 210||1.19048E-06||0.360750361||4.139249639 | |||
|} | |||
<br> | |||
==UV/vis of Pepsin-AuNP and HRP-AuNP== | |||
'''General Protocol''': | |||
# Place 1mL of each ratio in an 1.5mL eppendorf tube. | |||
# Centrifuge at maximum speed for 30 minutes. | |||
# Take a UV-Vis spectrum of each sample from 200nm to 800nm. | |||
# After taking the spectrum, store the sample in a fresh eppy tube in an upright position. | |||
<br> | |||
Figure 1. Corrected Absorbance spectra of pepsin-AuNPs | |||
<br> | |||
[[Image:Pepsin Nano.png]] | |||
<br> | |||
*Both 30:1 and 70:1 pepsin-AuNP had an absorbance peak at 518nm. | |||
==Notes== | |||
*The remaining UV/vis samples will be run during the next lab period. | |||
*Although neither the HRP or the pepsin solutions formed fibers, a black residue and color change(see figure 2) was observed for the pepsin solutions. The black residue will be tested in the future for identification. | |||
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Objectives
HRP-AuNP and Myoglobin-AuNP
UV/vis of Pepsin-AuNP and HRP-AuNPGeneral Protocol:
Notes
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