User:Moira M. Esson/Notebook/CHEM-571/2013/10/22

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(HRP-AuNP and Pepsin-AuNP)
(Objectives)
Line 8: Line 8:
==Objectives==
==Objectives==
#Determine the absorbance of BSA-AuNPs synthesized on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/16|10/16/2013]].
#Determine the absorbance of BSA-AuNPs synthesized on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/16|10/16/2013]].
-
#
+
#Synthesize Pepsin-AuNPs and HRP-AuNPs for catalytic activity testing
<br>
<br>
 +
==HRP-AuNP and Pepsin-AuNP==
==HRP-AuNP and Pepsin-AuNP==
<br>
<br>

Revision as of 13:34, 22 October 2013

Project name Main project page
Previous entry      Next entry

Objectives

  1. Determine the absorbance of BSA-AuNPs synthesized on 10/16/2013.
  2. Synthesize Pepsin-AuNPs and HRP-AuNPs for catalytic activity testing


HRP-AuNP and Pepsin-AuNP


Table 1. Preparation of Protein-AuNP solutions

Ratio Amount of gold added(mL) concentration HRP(M) concentration Pepsin(M) Volume HRP(mL) Volume Pepsin(mL) Volume Water HRP (mL) Volume Water Pepsin (mL)
300.4960317468.33333E-068.33333E-062.7233115472.6539278131.7806567071.850040441
500.4960317460.0000050.0000051.6339869281.5923566882.8699813262.911611566
700.4960317463.57143E-063.57143E-061.167133521.1373976343.3368347343.36657062
900.4960317462.77778E-062.77778E-060.9077705160.8846426043.5961977383.61932565
1100.4960317462.27273E-062.27273E-060.7427213310.7237984943.7612469233.780169759
1300.4960317461.92308E-061.92308E-060.6284565110.612444883.8755117433.891523374
1500.4960317461.66667E-061.66667E-060.5446623090.5307855633.9593059453.973182691
1700.4960317461.47059E-061.47059E-060.4805843910.4683402024.0233838634.035628052
1900.4960317461.31579E-061.31579E-060.429996560.4190412344.0739716944.08492702
2100.4960317461.19048E-061.19048E-060.3890445070.3791325454.1149237474.124835709


Personal tools