User:Moira M. Esson/Notebook/CHEM-571/2013/10/22

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Objectives)
(HRP-AuNP and Pepsin-AuNP)
Line 16: Line 16:
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Ratio'''
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount of gold added'''
| align="center" style="background:#f0f0f0;"|'''concentration HRP(M)'''
| align="center" style="background:#f0f0f0;"|'''concentration HRP(M)'''
| align="center" style="background:#f0f0f0;"|'''concentration Pepsin(M)'''
| align="center" style="background:#f0f0f0;"|'''concentration Pepsin(M)'''
Line 23: Line 24:
| align="center" style="background:#f0f0f0;"|'''Volume Water Pepsin (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Water Pepsin (mL)'''
|-
|-
-
| 30||8.33333E-06||8.33333E-06||2.723311547||2.653927813||1.780656707||1.850040441
+
| 30||0.496031746||8.33333E-06||8.33333E-06||2.723311547||2.653927813||1.780656707||1.850040441
|-
|-
-
| 50||0.000005||0.000005||1.633986928||1.592356688||2.869981326||2.911611566
+
| 50||0.496031746||0.000005||0.000005||1.633986928||1.592356688||2.869981326||2.911611566
|-
|-
-
| 70||3.57143E-06||3.57143E-06||1.16713352||1.137397634||3.336834734||3.36657062
+
| 70||0.496031746||3.57143E-06||3.57143E-06||1.16713352||1.137397634||3.336834734||3.36657062
|-
|-
-
| 90||2.77778E-06||2.77778E-06||0.907770516||0.884642604||3.596197738||3.61932565
+
| 90||0.496031746||2.77778E-06||2.77778E-06||0.907770516||0.884642604||3.596197738||3.61932565
|-
|-
-
| 110||2.27273E-06||2.27273E-06||0.742721331||0.723798494||3.761246923||3.780169759
+
| 110||0.496031746||2.27273E-06||2.27273E-06||0.742721331||0.723798494||3.761246923||3.780169759
|-
|-
-
| 130||1.92308E-06||1.92308E-06||0.628456511||0.61244488||3.875511743||3.891523374
+
| 130||0.496031746||1.92308E-06||1.92308E-06||0.628456511||0.61244488||3.875511743||3.891523374
|-
|-
-
| 150||1.66667E-06||1.66667E-06||0.544662309||0.530785563||3.959305945||3.973182691
+
| 150||0.496031746||1.66667E-06||1.66667E-06||0.544662309||0.530785563||3.959305945||3.973182691
|-
|-
-
| 170||1.47059E-06||1.47059E-06||0.480584391||0.468340202||4.023383863||4.035628052
+
| 170||0.496031746||1.47059E-06||1.47059E-06||0.480584391||0.468340202||4.023383863||4.035628052
|-
|-
-
| 190||1.31579E-06||1.31579E-06||0.42999656||0.419041234||4.073971694||4.08492702
+
| 190||0.496031746||1.31579E-06||1.31579E-06||0.42999656||0.419041234||4.073971694||4.08492702
|-
|-
-
| 210||1.19048E-06||1.19048E-06||0.389044507||0.379132545||4.114923747||4.124835709
+
| 210||0.496031746||1.19048E-06||1.19048E-06||0.389044507||0.379132545||4.114923747||4.124835709
|}
|}

Revision as of 13:33, 22 October 2013

Project name Main project page
Previous entry      Next entry

Objectives

  1. Determine the absorbance of BSA-AuNPs synthesized on 10/16/2013.


HRP-AuNP and Pepsin-AuNP


Table 1. Preparation of Protein-AuNP solutions

Ratio Amount of gold added concentration HRP(M) concentration Pepsin(M) Volume HRP(mL) Volume Pepsin(mL) Volume Water HRP (mL) Volume Water Pepsin (mL)
300.4960317468.33333E-068.33333E-062.7233115472.6539278131.7806567071.850040441
500.4960317460.0000050.0000051.6339869281.5923566882.8699813262.911611566
700.4960317463.57143E-063.57143E-061.167133521.1373976343.3368347343.36657062
900.4960317462.77778E-062.77778E-060.9077705160.8846426043.5961977383.61932565
1100.4960317462.27273E-062.27273E-060.7427213310.7237984943.7612469233.780169759
1300.4960317461.92308E-061.92308E-060.6284565110.612444883.8755117433.891523374
1500.4960317461.66667E-061.66667E-060.5446623090.5307855633.9593059453.973182691
1700.4960317461.47059E-061.47059E-060.4805843910.4683402024.0233838634.035628052
1900.4960317461.31579E-061.31579E-060.429996560.4190412344.0739716944.08492702
2100.4960317461.19048E-061.19048E-060.3890445070.3791325454.1149237474.124835709


Personal tools