User:Moira M. Esson/Notebook/CHEM-571/2013/10/22

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#Determine the absorbance of BSA-AuNPs synthesized on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/16|10/16/2013]].
#Determine the absorbance of BSA-AuNPs synthesized on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/16|10/16/2013]].
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<br>
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==HRP-AuNP and Pepsin-AuNP==
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<br>
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Table 1. Preparation of Protein-AuNP solutions
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''concentration HRP(M)'''
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| align="center" style="background:#f0f0f0;"|'''concentration Pepsin(M)'''
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| align="center" style="background:#f0f0f0;"|'''Volume HRP(mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Pepsin(mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Water HRP (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Water Pepsin (mL)'''
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|-
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| 30||8.33333E-06||8.33333E-06||2.723311547||2.653927813||1.780656707||1.850040441
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|-
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| 50||0.000005||0.000005||1.633986928||1.592356688||2.869981326||2.911611566
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|-
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| 70||3.57143E-06||3.57143E-06||1.16713352||1.137397634||3.336834734||3.36657062
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|-
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| 90||2.77778E-06||2.77778E-06||0.907770516||0.884642604||3.596197738||3.61932565
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|-
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| 110||2.27273E-06||2.27273E-06||0.742721331||0.723798494||3.761246923||3.780169759
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|-
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| 130||1.92308E-06||1.92308E-06||0.628456511||0.61244488||3.875511743||3.891523374
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|-
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| 150||1.66667E-06||1.66667E-06||0.544662309||0.530785563||3.959305945||3.973182691
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|-
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| 170||1.47059E-06||1.47059E-06||0.480584391||0.468340202||4.023383863||4.035628052
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|-
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| 190||1.31579E-06||1.31579E-06||0.42999656||0.419041234||4.073971694||4.08492702
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|-
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| 210||1.19048E-06||1.19048E-06||0.389044507||0.379132545||4.114923747||4.124835709
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|}
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Revision as of 12:27, 22 October 2013

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Objectives

  1. Determine the absorbance of BSA-AuNPs synthesized on 10/16/2013.


HRP-AuNP and Pepsin-AuNP


Table 1. Preparation of Protein-AuNP solutions

Ratio concentration HRP(M) concentration Pepsin(M) Volume HRP(mL) Volume Pepsin(mL) Volume Water HRP (mL) Volume Water Pepsin (mL)
308.33333E-068.33333E-062.7233115472.6539278131.7806567071.850040441
500.0000050.0000051.6339869281.5923566882.8699813262.911611566
703.57143E-063.57143E-061.167133521.1373976343.3368347343.36657062
902.77778E-062.77778E-060.9077705160.8846426043.5961977383.61932565
1102.27273E-062.27273E-060.7427213310.7237984943.7612469233.780169759
1301.92308E-061.92308E-060.6284565110.612444883.8755117433.891523374
1501.66667E-061.66667E-060.5446623090.5307855633.9593059453.973182691
1701.47059E-061.47059E-060.4805843910.4683402024.0233838634.035628052
1901.31579E-061.31579E-060.429996560.4190412344.0739716944.08492702
2101.19048E-061.19048E-060.3890445070.3791325454.1149237474.124835709


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