User:Moira M. Esson/Notebook/CHEM-571/2013/10/16: Difference between revisions
From OpenWetWare
Line 40: | Line 40: | ||
==UV/vis of Citrate and BSA samples== | ==UV/vis of Citrate and BSA samples== | ||
*A new BSA-Au sample was prepared because the protein had crashed out of solution. The BSA solution was prepared following the protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]]. The absorbance of the BSA solution will be determined during the next meeting. | *A new BSA-Au sample was prepared because the protein had crashed out of solution. The BSA solution was prepared following the protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]]. The absorbance of the BSA solution will be determined during the next meeting. | ||
Preparation of BSA stock solution | |||
0.025g BSA in 25mL tris buffer(described above)-->15μM BSA | |||
*A 1/10 dilution of Citrate-Au solution was measured using UV/vis | *A 1/10 dilution of Citrate-Au solution was measured using UV/vis | ||
<br> | <br> | ||
Figure 2. Absorbance spectra of 1/10 dilution of Citrate-AuNP | Figure 2. Absorbance spectra of 1/10 dilution of Citrate-AuNP and 1/10 dilution of 30:1 HRP-Au solution | ||
<br> | <br> | ||
[[Image:HRP and Citrate AuNP.png|750px]] | [[Image:HRP and Citrate AuNP.png|750px]] |
Revision as of 14:06, 21 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
Catalytic activity testing
Buffer:0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM Luminol:Dissolve 12.9mg luminol in 300uL of DMSO and add to 50mL buffer---> 1.46mM H2O2: 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM
Calculations for preparation of sample: Luminol stock solution preparation 5mL 7.26E-5 M luminol: 0.261mL luminol stock added to 5mL buffer. H2O2 stock preparation of 5mL 0.0038M(this is 50x more concentrated): 0.169mL stock in 5mL buffer.
UV/vis of Citrate and BSA samples
Preparation of BSA stock solution 0.025g BSA in 25mL tris buffer(described above)-->15μM BSA
|