User:Moira M. Esson/Notebook/CHEM-571/2013/10/16: Difference between revisions
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==Objectives== | ==Objectives== | ||
* Test the enzymatic/catalytic activity of HRP-AuNPs using luminol and UV/vis with the goal of determining the activity of HRP-AuNPs. | * Test the enzymatic/catalytic activity of HRP-AuNPs using luminol and UV/vis with the goal of determining the activity of HRP-AuNPs. | ||
* Calculate the absorbance prepared Citrate-Au solutions and BSA-Au solutions as standards (solutions were previously prepared on [[ | * Calculate the absorbance prepared Citrate-Au solutions and BSA-Au solutions as standards (solutions were previously prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]] | ||
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==Catalytic activity testing== | ==Catalytic activity testing== | ||
*Catalytic activity was tested using UV/vis spectroscopy. | *Catalytic activity was tested using UV/vis spectroscopy. |
Revision as of 13:59, 21 October 2013
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Objectives
Catalytic activity testing
Buffer:0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM Luminol:Dissolve 12.9mg luminol in 300uL of DMSO and add to 50mL buffer---> 1.46mM H2O2: 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM
Calculations for preparation of sample: #Luminol stock solution preparation 5mL 7.26E-5 M luminol: 0.261mL luminol stock added to 5mL buffer. #H2O2 stock preparation of 5mL 0.0038M(this is 50x more concentrated): 0.169mL stock in 5mL buffer.
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