User:Moira M. Esson/Notebook/CHEM-571/2013/10/15

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(Autocreate 2013/10/15 Entry for User:Moira_M._Esson/Notebook/CHEM-571)
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==Entry title==
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==Objectives==
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* Insert content here...
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* Synthesize four HRP-Au nanoparticle solutions for enzymatic study to be completed on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/16|10/17/2013]]
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<br>
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==General Protocol==
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*The synthesis of the HRP wrapped Au nanoparticles followed the general procedure described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]] for the preparation of BSA-AuNPs.
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*Four solutions of HRP-NPs were prepared:
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#10mL 30:1 Au-HRP (Final concentration of Au: 0.25mM)
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#10mL 60:1 Au-HRP (Final concentration of Au: 0.25mM)
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#10mL 90:1 Au-HRP (Final concentration of Au: 0.25mM)
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#10mL 120:1 Au-HRP(Final concentration of Au: 0.25mM)
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<br>
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Preparation of solutions:
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Standard solution concentrations- 2.94mM Au and 16.27μM HRP 
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  '''Solution 1: 8.5E-4L gold and 0.028mL HRP'''
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  '''Solution 2: 8.5E-4L gold and 0.014mL HRP'''
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  '''Solution 3: 8.5E-4L gold and 0.0094mL HRP'''
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  '''Solution 4: 8.5E-4L gold and 0.0071mL HRP'''
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<br>
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==Notes==
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*When first prepared, the solutions were all a brownish color. After heating, all of the solutions were a purplish color.
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*30:1 Au-HRP and 60:1 Au-HRP solutions contained dark black precipitate at the bottom of the sample. This may be excess gold.
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Revision as of 15:10, 21 October 2013

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Objectives

  • Synthesize four HRP-Au nanoparticle solutions for enzymatic study to be completed on 10/17/2013


General Protocol

  • The synthesis of the HRP wrapped Au nanoparticles followed the general procedure described on 08/28/2013 for the preparation of BSA-AuNPs.
  • Four solutions of HRP-NPs were prepared:
  1. 10mL 30:1 Au-HRP (Final concentration of Au: 0.25mM)
  2. 10mL 60:1 Au-HRP (Final concentration of Au: 0.25mM)
  3. 10mL 90:1 Au-HRP (Final concentration of Au: 0.25mM)
  4. 10mL 120:1 Au-HRP(Final concentration of Au: 0.25mM)



Preparation of solutions: Standard solution concentrations- 2.94mM Au and 16.27μM HRP

  Solution 1: 8.5E-4L gold and 0.028mL HRP
  Solution 2: 8.5E-4L gold and 0.014mL HRP
  Solution 3: 8.5E-4L gold and 0.0094mL HRP
  Solution 4: 8.5E-4L gold and 0.0071mL HRP


Notes

  • When first prepared, the solutions were all a brownish color. After heating, all of the solutions were a purplish color.
  • 30:1 Au-HRP and 60:1 Au-HRP solutions contained dark black precipitate at the bottom of the sample. This may be excess gold.



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