User:Moira M. Esson/Notebook/CHEM-571/2013/10/09: Difference between revisions
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*A new solution of 40μM adenosine solution was prepared following the protocol described on [[User:Moira_M._Esson/Notebook/CHEM-571/2013/10/08|2013/10/08]]. | *A new solution of 40μM adenosine solution was prepared following the protocol described on [[User:Moira_M._Esson/Notebook/CHEM-571/2013/10/08|2013/10/08]]. | ||
*2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette. | *2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette. | ||
*A more detailed protocol | *A more detailed protocol is described in [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/08|Matt Harting's]] notebook | ||
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Prepared solutions: | |||
*Note: The following solution was prepared by [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/08|Matt Harting's]] | |||
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EHNA stock | |||
5mg EHNA in 1mL DMSO ---> 15.9mM EHNA | |||
(1.9μL)(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA | |||
The reaction samples will contain roughly 1nM EHNA | |||
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'''UV/vis''' | |||
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Revision as of 18:57, 12 October 2013
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Objectives
KineticsGeneral Protocol:
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