User:Moira M. Esson/Notebook/CHEM-571/2013/10/01: Difference between revisions
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#Luminol: 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8 | #Luminol: 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8 | ||
#Buffer: 5mM Tris pH=8 | #Buffer: 5mM Tris pH=8 | ||
#HRP: 1.7μM HRP in 50mL 5mM Tris pH=8 | #HRP: 1.7μM HRP in 50mL 5mM Tris pH=8 | ||
#H<sub>2</sub>O<sub>2</sub>: 117μL of 30%H<sub>2</sub>O<sub>2</sub> in 50mL 5mM buffer. | |||
'''Samples Run:''' | |||
#1/20 dilution of lumino1 | |||
#HRP stock solution | |||
#Reaction of HRP+Luminol stock+H<sub>2</sub>O<sub>2</sub> (prepared solution by taking 1/3mL of each sample and allowing to react for ~5min) | |||
<br> | |||
'''Sample notes:''' | |||
*Performed 1/20 dilution of 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8. (The luminol stock solution). | *Performed 1/20 dilution of 13.5mg luminol in 300μL DMSO added to 50mL 5mM Tris pH=8. (The luminol stock solution). | ||
**Make a 10mL stock solution of 1/20 dilute luminol stock solution. | **Make a 10mL stock solution of 1/20 dilute luminol stock solution. | ||
*Prepared stock solution of hydrogen peroxide was too dilute to measure (characteristic peak at 432 nm was not present) | *Prepared stock solution of hydrogen peroxide was too dilute to measure (characteristic peak at 432 nm was not present) | ||
Revision as of 11:33, 1 October 2013
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Objectives
UV-visStock solutions prepared before class:
Samples Run:
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