Objectives
- Perform error analysis on the compiled data calibration curve.
- Determine the standard deviation for the data points.
- Determine the confidence interval for 90% and 95% confidence.
- Determine if any data can be ruled out using a Q-test.
- Determine the molar extinction coefficient for both adenosine and inosine.
- Determine the concentration of an unknown adenosine sample(taken from Javier's group).
- Perform Grubb's test on outlying peak of adenosine molar extinction coefficent graph.
- Perform data analysis on molar extinction coefficient graphs(both adenosine and inosine).
Group calibration curve data
Figure 1. CHEM-571 Calibration Curve for Adenosine
- The Grubb's test will be employed to determine whether the points that lie outside of the line of best fit are outliers
Figure 2. CHEM-571 Calibration Curve for Inosine
Table 1. Molar absorptivity for Adenosine and Inosine
| Molar Absorptivity(Lmol-1cm-1)
|
'
|
| Adenosine |
14025
|
| Inosine |
11007
|
Table 2. Standard deviation of Inosine Absorbance values
| Comcentration(M)
|
Standard Deviation
|
| 4.80E-05 |
0.10707708
|
| 4.00E-05 |
0.12254917
|
| 3.20E-05 |
0.0943149
|
| 2.40E-05 |
0.06671357
|
| 1.60E-05 |
0.04841797
|
| 8.00E-06 |
0.04748368
|
Table 3. Standard deviation of Adenosine Absorbance values
| Concentration(M)
|
Standard Deviation
|
| 3.00E-05 |
0.072537576
|
| 2.50E-05 |
0.045680412
|
| 2.00E-05 |
0.064123319
|
| 1.50E-05 |
0.056753854
|
| 1.00E-05 |
0.0313289
|
| 5.00E-06 |
0.046559639
|
Unknown Adenosine Solution
Figure 3. Absorption Spectrum of Unknown Concentration
- Using the molar extinction coefficient determined from the calibration curve above the concentration of the unknown is 4.7344E-5 M.
| 
Project name
