User:Moira M. Esson/Notebook/CHEM-571/2013/09/04

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Objectives

  • Perform error analysis on the compiled data calibration curve.
    • Determine the standard deviation for your data points.
    • Determine the confidence interval for 90% and 95% confidence.
    • Determine if any data can be ruled out using a Q-test.
  • Determine the molar extinction coefficient for both adenosine and inosine.
  • Determine the concentration of an unknown adenosine sample(taken from Javier's group).
  • Perform Grubb's test on outlying peak of adenosine molar extinction coefficent graph.
  • Perform data analysis on molar extinction coefficient graphs(both adenosine and inosine).


Group calibration curve data

Figure 1. CHEM-571 Calibration Curve for Adenosine

  • The Grubb's test will be employed to determine whether the points that lie outside of the line of best fit are outliers


Figure 2. CHEM-571 Calibration Curve for Inosine

Unknown Adenosine Solution

Figure 3. Absorption Spectrum of Unknown Concentration

  • Using the molar extinction coefficient determined from the calibration curve above the concentration of the unknown is 4.7344E-5 M.