User:Moira M. Esson/Notebook/CHEM-571/2013/09/04: Difference between revisions

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| Inosine||11007
| Inosine||11007
|}
|}
<br>
'''Table 2. Standard deviation of Inosine Absorbance values'''
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Comcentration(M)'''
| align="center" style="background:#f0f0f0;"|'''Standard Deviation'''
|-
| 4.80E-05||0.10707708
|-
| 4.00E-05||0.12254917
|-
| 3.20E-05||0.0943149
|-
| 2.40E-05||0.06671357
|-
| 1.60E-05||0.04841797
|-
| 8.00E-06||0.04748368
|}
<br>
'''Table 3. Standard deviation of Adenosine Absorbance values'''
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Concentration(M)'''
| align="center" style="background:#f0f0f0;"|'''Standard Deviation'''
|-
| 3.00E-05||0.072537576
|-
| 2.50E-05||0.045680412
|-
| 2.00E-05||0.064123319
|-
| 1.50E-05||0.056753854
|-
| 1.00E-05||0.0313289
|-
| 5.00E-06||0.046559639
|}
<br>


==Unknown Adenosine Solution==
==Unknown Adenosine Solution==

Revision as of 20:03, 8 September 2013

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Objectives

  • Perform error analysis on the compiled data calibration curve.
    • Determine the standard deviation for the data points.
    • Determine the confidence interval for 90% and 95% confidence.
    • Determine if any data can be ruled out using a Q-test.
  • Determine the molar extinction coefficient for both adenosine and inosine.
  • Determine the concentration of an unknown adenosine sample(taken from Javier's group).
  • Perform Grubb's test on outlying peak of adenosine molar extinction coefficent graph.
  • Perform data analysis on molar extinction coefficient graphs(both adenosine and inosine).


Group calibration curve data

Figure 1. CHEM-571 Calibration Curve for Adenosine

  • The Grubb's test will be employed to determine whether the points that lie outside of the line of best fit are outliers


Figure 2. CHEM-571 Calibration Curve for Inosine

Table 1. Molar absorptivity for Adenosine and Inosine

Molar Absorptivity(Lmol-1cm-1) '
Adenosine 14025
Inosine 11007


Table 2. Standard deviation of Inosine Absorbance values

Comcentration(M) Standard Deviation
4.80E-05 0.10707708
4.00E-05 0.12254917
3.20E-05 0.0943149
2.40E-05 0.06671357
1.60E-05 0.04841797
8.00E-06 0.04748368


Table 3. Standard deviation of Adenosine Absorbance values

Concentration(M) Standard Deviation
3.00E-05 0.072537576
2.50E-05 0.045680412
2.00E-05 0.064123319
1.50E-05 0.056753854
1.00E-05 0.0313289
5.00E-06 0.046559639


Unknown Adenosine Solution

Figure 3. Absorption Spectrum of Unknown Concentration

  • Using the molar extinction coefficient determined from the calibration curve above the concentration of the unknown is 4.7344E-5 M.



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