User:Moira M. Esson/Notebook/CHEM-571/2013/09/04: Difference between revisions

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| align="center" style="background:#f0f0f0;"|'''Molar Absorptivity(L*mol<sup>-1</sup>*cm<sup>-1</sup>'''
| align="center" style="background:#f0f0f0;"|'''Molar Absorptivity(Lmol<sup>-1</sup>cm<sup>-1</sup>)'''
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Revision as of 19:26, 8 September 2013

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Objectives

  • Perform error analysis on the compiled data calibration curve.
    • Determine the standard deviation for your data points.
    • Determine the confidence interval for 90% and 95% confidence.
    • Determine if any data can be ruled out using a Q-test.
  • Determine the molar extinction coefficient for both adenosine and inosine.
  • Determine the concentration of an unknown adenosine sample(taken from Javier's group).
  • Perform Grubb's test on outlying peak of adenosine molar extinction coefficent graph.
  • Perform data analysis on molar extinction coefficient graphs(both adenosine and inosine).


Group calibration curve data

Figure 1. CHEM-571 Calibration Curve for Adenosine

  • The Grubb's test will be employed to determine whether the points that lie outside of the line of best fit are outliers


Figure 2. CHEM-571 Calibration Curve for Inosine

Table 1. Molar absorptivity for Adenosine and Inosine

Molar Absorptivity(Lmol-1cm-1) '
Adenosine 14025
Inosine 11007

Unknown Adenosine Solution

Figure 3. Absorption Spectrum of Unknown Concentration

  • Using the molar extinction coefficient determined from the calibration curve above the concentration of the unknown is 4.7344E-5 M.



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