Objectives
- Prepare adenosine solutions of various concentrations to determine the molar absorptivity of adenosine.
- Prepare inosine solutions of various concentrations to determine the molar absorptivity of inosine.
- Prepare calibration curves for adenosine and inosine.
- Analyze the data of all of the combined CHEM-571 data.
Preparation of Adenosine Stock solution
General Protocol
- In a clean 50mL volumetric flask, add 0.200g adenosine. Fill the volumetric flask with deionized H2O up until the white line.
- Note: To ensure the full addition of all of the adenosine, a small amount of deionized H2O was placed on the weigh boat and a pipette was used to remove all of the H2O on the weigh boat.s
- Cap the volumetric flask and shake the volumetric flask vigorously until the adenosine is completely dissolved.
- Note:The solution should be shaken vigorously for a minimum of 5 minutes.
- Pour the solution into a 50mL falcon tube for later storage.
- Dilute the solution by taking the necessary volume of the solution (using an automated pipette), placing the solution into the appropriately sized volumetric flask, and add deionized H2O.
- Needed Stock Solutions for Adenosine
Adenosine solution concentrations (M)
|
3.00x10-5
|
2.50x10-5
|
2.00x10-5
|
1.50x10-5
|
1.00x10-5
|
0.50x10-5
|
RANDOM
|
- Stock Solution Preparation for Adenosine
Total Mass [g]
|
Stock Volume [mL]
|
Concentration [M]
|
0.2006 |
50 |
0.015 |
|
- Serial Dilution of Adenosine Stock Solution
Dilution
|
'M1'[mol/L]
|
'V1' [mL]
|
'M2'[mol/L]
|
'V2' [mL]
|
1 |
0.015 |
0.1 |
1.5E-4 |
10 |
|
2 |
0.015 |
0.25 |
3.0E-5 |
10 |
|
3 |
1.5E-4 |
1.667 |
2.5E-5 |
10 |
|
4 |
1.5E-4 |
1.333 |
2.0E-5 |
10 |
|
5 |
1.5E-4 |
1.0 |
1.5E-5 |
10 |
|
6 |
1.5E-4 |
0.667 |
1.0E-5 |
10 |
|
7 |
1.5E-4 |
0.333 |
0.5E-5 |
10 |
|
8 |
1.5E-4 |
0.5 |
0.75E-5 |
10 |
|
Preparation of Inosine Stock Solutions
General Protocol
- In a clean 25mL volumetric flask, add 0.1347g inosine. Fill the volumetric flask with deionized H2O up until the white line.
- Note: To ensure the full addition of all of the inosine, a small amount of deionized H2O was placed on the weigh boat and a pipette was used to remove all of the H2O on the weigh boat.s
- Cap the volumetric flask and shake the volumetric flask vigorously until the inosine is completely dissolved.
- Note:The solution should be shaken vigorously for a minimum of 3 minutes.
- Pour the solution into a 50mL falcon tube for later storage.
- Dilute the solution by taking the necessary volume of the solution (using an automated pipette), placing the solution into the appropriately sized volumetric flask, and add deionized H2O.
- Needed Concentrations for Inosine Solutions
Inosine solution concentrations (M)
|
4.80x10-5
|
4.00x10-5
|
3.20x10-5
|
2.40x10-5
|
1.60x10-5
|
0.80x10-5
|
RANDOM
|
- Stock Solution Preparation for Inosine
Total Mass [g]
|
Stock Volume [mL]
|
Concentration [M]
|
0.1347 |
25 |
0.02 |
|
- Serial Dilution of Inosine Stock Solution
Dilution
|
'M1'[mol/L]
|
'V1' [mL]
|
'M2'[mol/L]
|
'V2' [mL]
|
1 |
0.02 |
0.24 |
4.8E-4 |
10 |
|
2 |
4.8E-4 |
1.0 |
4.8E-5 |
10 |
|
3 |
4.8E-4 |
0.833 |
4.0E-5 |
10 |
|
4 |
4.8E-4 |
0.667 |
3.2E-5 |
10 |
|
5 |
4.8E-4 |
0.5 |
2.4E-5 |
10 |
|
6 |
4.8E-4 |
0.333 |
1.6E-5 |
10 |
|
7 |
4.8E-4 |
0.1667 |
0.8E-5 |
10 |
|
8 |
1.5E-4 |
0.25 |
1.2E-5 |
10 |
|
UV/vis
Notes
- Originally the adenosine stock solution was prepared as 0.03M in 25mL. However, the adenosine was not soluble in such a small volume so the original adenosine solution was poured into a 50mL volumetric flask and rinsed throughly with deionized H2O.
|